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Comparison of the Chiron ultrasensitive bDNA and original bDNA assays for the measurement of HIV RNA in plasma samples

  1. Author:
    Highbarger, H.
    Jiang, M.
    Shaw, A.
    Davis, I.
    Lane, H. C.
    Dewar, R.
    1. Year of Conference: 1997
  2. Conference Name: Conference on Retroviruses and Opportunistic Infections
    1. Pages: 179 (abstract no. 618)
  4. Type of Work: Meeting Abstract
  1. Abstract:

    Objective. In order to determine whether Chiron's new ultrasensitive bDNA assay was measuring the same phenomenon of viral RNA in plasma as their original bDNA assay, duplicate samples of plasma were run on both assays. The two assays yield comparable data which allows the reporting of viral load down to 500 copies as opposed to the 10,000 copy cutoff of the older assay. Methods. Frozen plasma samples (569) from 287 patients on various drug protocols at NIH's outpatient HIV clinic were tested in duplicate on both the ultrasensitive and the original Chiron bDNA assays. Results. Of the 569 samples assayed, 190 (33%) fell into the less than 10,000 copies of RNA/MI or undetectable range by regular bDNA but were detectable by the ultrasensitive assay, 240 (42%) were undetectable by both assays, and 4 (.007%) were detectable by the regular bDNA but not by the ultrasensitive assay. For samples that were detectable by both assays, the regular and ultrasensitive values showed a correllation coefficient of 0.80. Discussion. It is now possible, with Chiron's ultrasensitive assay, to give clinicians more sensitive data. Whether or not this will affect management of patient care depends on how this information is used and merits more study.

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