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On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: Evaluation for peptide analysis and protein identification

  1. Author:
    Janini, G. M.
    Zhou, M.
    Yu, L. R.
    Blonder, J.
    Gignac, M.
    Conrads, T. P.
    Issaq, H. J.
    Veenstra, T. D.

  2. Author Address

    NCI, Lab Proteom & Analyt Technol, SAIC Frederick Inc, POB B, Frederick, MD 21702 USA NCI, Lab Proteom & Analyt Technol, SAIC Frederick Inc, Frederick, MD 21702 USA NCI, Image Anal Lab, SAIC Frederick Inc, Frederick, MD 21702 USA Janini GM NCI, Lab Proteom & Analyt Technol, SAIC Frederick Inc, POB B, Frederick, MD 21702 USA
    1. Year: 2003
  2. Journal: Analytical Chemistry
    1. 75
    2. 21
    3. Pages: 5984-5993
  4. Type of Article: Article
  1. Abstract:

    Although several designs have been advanced for coupling sample enrichment devices to a sheathless electrospray ionization-mass spectrometry (MS) interface on a capillary electrophoresis (CE) column, most of these approaches suffer from difficulties in fabrication, and the CE separation efficiency is degraded as a result of the presence of coupling sleeves. We have developed a design that offers significant improvements in terms of ease of fabrication, durability, and maintenance of the integrity of the CE-separated analyte zones. Capillaries with different inside and outside diameters were evaluated to optimize the performance of the CE-MS system, resulting in a mass limit of detection of 500 amol for tandem MS analysis of a standard peptide using a 20-mum-i.d. capillary. The improved design incorporates an efficient method to preconcentrate a sample directly within the CE capillary followed by its electrophoretic separation and detection using a true zero dead-volume sheathless CE-MS interface. Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides.

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