Skip NavigationSkip to Content
Return Return to Search Results

Effects of electron-beam irradiation on buccal-cell DNA

  1. Author:
    Castle, P. E.
    Garcia-Closas, M.
    Franklin, T.
    Chanock, S.
    Puri, V.
    Welch, R.
    Rothman, N.
    Vaught, J.

  2. Author Address

    NCI, Div Canc Epidemiol & Genet, NIH, US Dept HHS, 6120 Execut Blvd,Room 7074,EPS MSC 7234, Bethesda, MD 20892 USA NCI, Div Canc Epidemiol & Genet, NIH, US Dept HHS, Bethesda, MD 20892 USA NCI, Pediat Oncol Branch, Ctr Canc Res, NIH,US Dept HHS, Bethesda, MD 20892 USA Amer Type Culture Collect, Cell Biol, Manassas, VA USA NCI, Core Genotyping Facil, Ctr Adv Technol, Gaithersburg, MD USA NCI, Intramural Res Support Program, Sci Applicat Int Corp Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21701 USA Castle PE NCI, Div Canc Epidemiol & Genet, NIH, US Dept HHS, 6120 Execut Blvd,Room 7074,EPS MSC 7234, Bethesda, MD 20892 USA
    1. Year: 2003
  2. Journal: American Journal of Human Genetics
    1. 73
    2. 3
    3. Pages: 646-651
  4. Type of Article: Article
  1. Abstract:

    Buccal cells were collected from 29 participants, by use of mouthwash rinses, and were split into equal aliquots, with one aliquot irradiated by electron-beam (E-beam) irradiation equivalent to the sterilizing dosage used by the U. S. Postal Service and the other left untreated. Aliquots were extracted and tested for DNA yields (e.g., TaqMan assay for quantifying human genomic DNA), genomic integrity, and amplification-based analysis of genetic variants (e.g., single-nucleotide polymorphisms [SNPs] and single tandem repeats [STRs]). Irradiated aliquots had lower median DNA yields (3.7 mug/aliquot) than untreated aliquots (7.6 mug/aliquot) (P < .0005) and were more likely to have smaller maximum DNA fragment size, on the basis of genomic integrity gels, than untreated aliquots (P < .0005). Irradiated aliquots showed poorer PCR amplification of a 989- bp beta-globin target ( 97% for weak amplification and 3% for no amplification) than untreated aliquots (7% for weak amplification and 0% for no amplification) (P < .0005), but 536-bp and 268-bp β-globin targets were amplified from all aliquots. There was no detectable irradiation effect on SNP assays, but there was a significant trend for decreased detection of longer STRs (P = .01) in irradiated versus untreated aliquots. We conclude that E-beam irradiation reduced the yield and quality of buccal-cell specimens, and, although irradiated buccal-cell specimens may retain sufficient DNA integrity for some amplified analyses of many common genomic targets, assays that target longer DNA fragments (>989 bp) or require whole-genome amplification may be compromised.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel