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Matrix Glycoprotein Sc1/Ecm2 Augments B Lymphopoiesis

  1. Author:
    Oritani, K.
    Kanakura, Y.
    Aoyama, K.
    Yokota, T.
    Copeland, N. G.
    Gilbert, D. J.
    Jenkins, N. A.
    Tomiyama, Y.
    Matsuzawa, Y.
    Kincade, P. W.

  2. Author Address

    Oritani K OSAKA UNIV SCH MED DEPT INTERNAL MED 2 2-2 YAMADAOKA SUITA OSAKA 565 JAPAN OSAKA UNIV SCH MED DEPT HEMATOL ONCOL SUITA OSAKA 565 JAPAN NCI FREDERICK CANC RES & DEV CTR MAMMALIAN GENET LAB ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA
    1. Year: 1997
  2. Journal: Blood
    1. 90
    2. 9
    3. Pages: 3404-3413
  4. Type of Article: Article
  1. Abstract:

    The extracellular matrix produced by stromal cells plays a critical role in lympho-hematopoiesis. It was recently discovered that matrix glycoprotein SC1/ECM2 is a component of that matrix and preliminary evidence suggested that it could contribute to the nurturing environment for B-lymphocyte precursors. A fusion protein prepared from the amino terminal portion of SC1/ECM2 and the constant region of human Ig preferentially bound to pre-B cells, Furthermore, the cloning efficiency of interleukin-7-dependent B cell precursors was increased in a dose-dependent manner by addition of this fusion protein, We now report the complete cDNA sequence for murine SC1/ECM2 and its localization to the central region of chromosome 5. A fusion protein prepared from the full length of SC1/EGM2 and Ig was found to recognize pre-a cells in a divalent cation dependent manner, and to augment mitogen-dependent proliferation of mature B cells, as well as the cloning of pre-B cells, but to have no influence on myeloid progenitor cells, Although SC1/ECM2 is normally a secreted protein, we show that it is also capable of augmenting lymphopoiesis when expressed as a transmembrane protein on fibroblasts. Although the C-terminal portion of SC1/ECM2 has sequence homology to osteonectin/ SPARC, the unique hi-terminal one fifth of the protein was sufficient to augment lymphocyte growth. (C) 1997 by The American Society of Hematology. [References: 45]

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