Skip NavigationSkip to Content
Return Return to Search Results

Evaluation of antimitotic agents by quantitative comparisons of their effects on the polymerization of purified tubulin

  1. Author:
    Hamel, E.

  2. Author Address

    NCI, Screening Technol Branch, Dev Therapeut Program, Div Canc Treatment & Diag,NIH, Frederick, MD 21702 USA NCI, Screening Technol Branch, Dev Therapeut Program, Div Canc Treatment & Diag,NIH, Frederick, MD 21702 USA Hamel E NCI, Screening Technol Branch, Dev Therapeut Program, Div Canc Treatment & Diag,NIH, Frederick, MD 21702 USA
    1. Year: 2003
  2. Journal: Cell Biochemistry and Biophysics
    1. 38
    2. 1
    3. Pages: 1-21
  4. Type of Article: Article
  1. Abstract:

    Most antimitotic compounds have highly specific interactions with tubulin, the major protein component of microtubules. It is, therefore, often desirable to characterize interactions of these agents with tubulin. In particular, quantitative comparisons between new and old ("standard") agents, between different classes of agent, and between structural analogs (e.g., for a structure-activity relationship study) are important. Because antimitotic drugs have a variety of effects on tubulin and bind at multiple distinct sites on the protein, the tubulin assembly reaction is probably the only universally applicable reaction that can be analyzed. In my laboratory, we use the assembly of purified tubulin induced by higher concentrations of monosodium glutamate as our basic assay system. This report presents a detailed description of our current routine assay, including the effects of a variety of reaction components on the reaction. In addition, the variety of effects that reaction components can have on the quantitative results obtained with drugs, using the colchicine site drug combretastatin A-4 as a model compound, is described.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel