Skip NavigationSkip to Content
Return Return to Search Results

GLIS3, a novel member of the GLIS subfamily of Kruppel-like zinc finger proteins with repressor and activation functions

  1. Author:
    Kim, Y. S.
    Nakanishi, G.
    Lewandoski, M.
    Jetten, A. M.

  2. Author Address

    NIEHS, Cell Biol Sect, Div Intramural Res, NIH,Dept Hlth & Human Serv, POB 12233, Res Triangle Pk, NC 27709 USA NIEHS, Cell Biol Sect, Div Intramural Res, NIH,Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA NCI, Lab Canc & Dev Biol, NIH, Frederick, MD 21702 USA Jetten AM NIEHS, Cell Biol Sect, Div Intramural Res, NIH,Dept Hlth & Human Serv, POB 12233, Res Triangle Pk, NC 27709 USA
    1. Year: 2003
  2. Journal: Nucleic Acids Research
    1. 31
    2. 19
    3. Pages: 5513-5525
  4. Type of Article: Article
  1. Abstract:

    In this study, we describe the identification and characterization of a novel transcription factor GLI-similar 3 (GLIS3). GLIS3 is an 83.8 kDa nuclear protein containing five C2H2-type Kruppel-like zinc finger motifs that exhibit 93% identity with those of GLIS1, however, little homology exists outside their zinc finger domains. GLIS3 can function as a repressor and activator of transcription. Deletion mutant analysis determined that the N- and C-termini are required for optimal transcriptional activity. GLIS3 binds to the GLI-RE consensus sequence and is able to enhance GLI-RE-dependent transcription. GLIS3(DeltaC496), a dominant-negative mutant, inhibits transcriptional activation by GLIS3 and GLI1. Whole mount in situ hybridization on mouse embryos from stage E6.5 through E14.5 demonstrated that GLIS3 is expressed in specific regions in developing kidney and testis and in a highly dynamic pattern during neurulation. From E11.5 through E12.5 GLIS3 was strongly expressed in the interdigital regions, which are fated to undergo apoptosis. The temporal and spatial pattern of GLIS3 expression observed during embryonic development suggests that it may play a critical role in the regulation of a variety of cellular processes during development. Both the repressor and activation functions of GLIS3 may be involved in this control.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel