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Transcriptome analysis of mouse stem cells and early embryos

  1. Author:
    Sharov, A. A.
    Piao, Y. L.
    Matoba, R.
    Dudekula, D. B.
    Qian, Y.
    Vanburen, V.
    Falco, G.
    Martin, P. R.
    Stagg, C. A.
    Bassey, U. C.
    Wang, Y. X.
    Carter, M. G.
    Hamatani, T.
    Aiba, K.
    Akutsu, H.
    Sharova, L.
    Tanaka, T. S.
    Kimber, W. L.
    Yoshikawa, T.
    Jaradat, S. A.
    Pantano, S.
    Nagaraja, R.
    Boheler, K. R.
    Taub, D.
    Hodes, R. J.
    Longo, D. L.
    Schlessinger, D.
    Keller, J.
    Klotz, E.
    Kelsoe, G.
    Umezawa, A.
    Vescovi, A. L.
    Rossant, J.
    Kunath, T.
    Hogan, B. L. M.
    Curci, A.
    D'Urso, M.
    Kelso, J.
    Hide, W.
    Ko, M. S. H.

  2. Author Address

    Ko, MSH, NIA, Baltimore, MD 21224 USA NIA, Baltimore, MD 21224 USA. NCI, Bethesda, MD 20892 USA. NCI, SAIC Frederick, Basic Res Program, Frederick, MD 21701 USA. Duke Univ, Ctr Med, Durham, NC USA. Natl Res Inst Child Hlth & Dev, Tokyo, Japan. Osped San Raffaele, Inst Stem Cell Res, I-20127 Milan, Italy. Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada. CNR, Inst Genet & Biophys, I-80125 Naples, Italy. Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Bellville, South Africa.
    1. Year: 2003
  2. Journal: Plos Biology
    1. 1
    2. 3
    3. Pages: 410-419
  4. Type of Article: Article
  1. Abstract:

    Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine

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