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Development of multiplex DNA electronic microarrays using a universal adaptor system for detection of single nucleotide polymorphisms

  1. Author:
    Tsang, S.
    Sun, Z. H.
    Stewart, C.
    Lum, N.
    Frankenberger, C.
    Subleski, M.
    Rasmussen, L.
    Munroe, D. J.

  2. Author Address

    Tsang, S, Lab Mol Technol, 915 Toll House Ave,Suite 211, Frederick, MD 21702 USA Natl Canc Inst, SAIC Frederick, Frederick, MD USA.
    1. Year: 2004
  2. Journal: Biotechniques
    1. 36
    2. 4
    3. Pages: 682-688
  4. Type of Article: Article
  1. Abstract:

    The NanoChip(R) electronic microarray is designed for the rapid detection of genetic variation in research and clinical diagnosis. We have developed a multiplex electronic microarray assay, specific for single nucleotide polymorphism (SNP) genotyping and mutation detection, using universal adaptor sequences tailed to the 5' end of PCR primers specific to each target. PCR products, amplified by primers directed to the universal adaptor sequence, are immobilized on the microarray either directly or via capture oligonucleotides complementary to the universal adaptor sequence. This simple modification results in a significant increase infidelity with improved specificity and accuracy. In addition, the multiplexing of genetic variant detection allows increased throughput and significantly reduced cost per assay. This general schema can also be applied to other microarray and macroarray formats

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