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Cenp-E Is an Essential Kinetochore Motor in Maturing Oocytes and Is Masked During Mos-Dependent, Cell Cycle Arrest At Metaphase Ii

  1. Author:
    Duesbery, N. S.
    Choi, T. S.
    Brown, K. D.
    Wood, K. W.
    Resau, J.
    Fukasawa, K.
    Clevel, D. W.
    Vande Woude, G. F

  2. Author Address

    Vandewoude GF NCI FREDERICK CANC RES & DEV CTR ABL BASIC RES PROGRAM POB B FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA JOHNS HOPKINS UNIV SCH MED DEPT BIOL CHEM BALTIMORE, MD 21205 USA UNIV CALIF SAN DIEGO LA JOLLA, CA 92093 USA LUDWIG INST CANC RES LA JOLLA, CA 92093 USA
    1. Year: 1997
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 94
    2. 17
    3. Pages: 9165-9170
  4. Type of Article: Article
  1. Abstract:

    CENP-E, a kinesin-like protein that is known to associate with kinetochores during all phases of mitotic chromosome movement, is shown here to be a component of meiotic kinetochores as well, CENP-E is detected at kinetochores during metaphase I in both mice and frogs, and, as in mitosis, is relocalized to the midbody during telophase, CENP-E function is essential for meiosis I because injection of an antibody to CENP-E into mouse oocytes in prophase completely prevented progression of those oocytes past metaphase I, Beyond this, CENP-E is modified or masked during the natural, Mos-dependent, cell cycle arrest that occurs at metaphase II, although it is readily detectable at the kinetochores in metaphase II oocytes derived from mos-deficient (MOS-/-) mice that fail to arrest at metaphase II, This must reflect a masking of some CENP-E epitopes, not the absence of CENP-E, in meiosis II because a different polyclonal antibody raised to the tail of CENP-E detects CENP-E at kinetochores of metaphase II-arrested eggs and because CENP-E reappears in telophase of mouse oocytes activated in the absence of protein synthesis. [References: 53]

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