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Sensitive phenotypic detection of minor drug-resistant human immunodeficiency virus type 1 reverse transcriptase variants

  1. Author:
    Nissley, D. V.
    Halvas, E. K.
    Hoppman, N. L.
    Garfinkel, D. J.
    Mellors, J. W.
    Strathern, J. N.

  2. Author Address

    SAIC Frederick Inc, NCI, Basic Res Program, Ft Detrick, MD 21702 USA. Univ Pittsburgh, Sch Med, Div Infect Dis, Pittsburgh, PA USA. NCI, Gene Regulat & Chromosome Biol Lab, Frederick, MD 21701 USA Nissley, DV, SAIC Frederick Inc, NCI, Basic Res Program, POB B, Ft Detrick, MD 21702 USA
    1. Year: 2005
    2. Date: NOV
  2. Journal: Journal of Clinical Microbiology
    1. 43
    2. 11
    3. Pages: 5696-5704
  4. Type of Article: Article
  1. Abstract:

    Detection of drug-resistant variants is important for the clinical management of human immunodeficiency virus type 1 (HIV-1) infection and for studies on the evolution of drug resistance. Here we show that hybrid elements composed of the Saccharomyces cerevisiae retrotransposon Ty1 and the reverse transcriptase (RT) of HIV-1 are useful tools for detecting, monitoring, and isolating drug-resistant reverse transcriptases. This sensitive phenotypic assay is able to detect nonnucleoside reverse transcriptase inhibitor-resistant RT domains derived from mixtures of infectious molecular clones of HIV-1 in plasma and from clinical samples when the variants comprise as little as 0.3 to 1% of the virus population. Our assay can characterize the activities and drug susceptibilities of both known and novel reverse transcriptase variants and should prove useful in studies of the evolution and clinical significance of minor drug-resistant viral variants

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External Sources

  1. View record in Web of Science®
  2. WOS: 000233312200045

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