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Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation

  1. Author:
    McAvin, J. C.
    Escamilla, E. M.
    Blow, J. A.
    Turell, M. J.
    Quintana, M.
    Bowles, D. E.
    Swaby, J. A.
    Barnes, W. J.
    Huff, W. B.
    Lohman, K. L.
    Atchley, D. H.
    Hickman, J. R.
    Niemeyer, D. M.

  2. Author Address

    USAF, Inst Environm Safety & Occupat Hlth Anal, Epidemiol Surveillance Div, San Antonio, TX 78235 USA. USA, Med Res Inst Infect Dis, Div Virol, Frederick, MD 21702 USA. USA, Ctr Hlth Promot & Prevent Med W, Div Environm Sci, Ft Lewis, WA 98433 USA. USAF, Force Battlelab, Lackland Air Force Base, San Antonio, TX 78236 USA. USAF, Off Surg Gen, Bolling Air Force Base, Washington, DC 20332 USA. Joint Program Execut Off Chem & Biol Def, Falls Church, VA 22041 USA.;McAvin, JC, USAF, Inst Environm Safety & Occupat Hlth Anal, Epidemiol Surveillance Div, San Antonio, TX 78235 USA.
    1. Year: 2005
    2. Date: Dec
  2. Journal: Military Medicine
    1. 170
    2. 12
    3. Pages: 1053-1059
  4. Type of Article: Article
  5. ISSN: 0026-4075
  1. Abstract:

    Dengue virus universal and dengue serotype 1 to 4, fluorogenic probe hydrolysis (TaqMan), reverse transcription-polymerase chain reaction assays were developed for screening and serotype identification of infected mosquito vectors and human sera using a field-deployable, fluorometric thermocycler. Dengue universal and dengue 1 to 4 serotype assay in vitro sensitivity and specificity results were 100% concordant when tested with total nucleic acid extracts of multiple strains of dengue serotype 1 to 4, yellow fever, Japanese encephalitis, West Nile, and St. Louis encephalitis viruses. The in vitro sensitivity and specificity results for all five assays were concordant when tested with a blind panel of 27 dengue virus-infected mosquitoes, 21 non-dengue (yellow fever, West Nile, or St. Louis encephalitis) flavivirus-infected mosquitoes, and 11 uninfected mosquitoes and with clinical specimens consisting of a human serum panel of eight dengue viremic and 31 non-dengue-infected febrile patient serum samples. No cross-reaction occurred with vector species or human genomic DNA. Sample processing and polymerase chain reaction required < 2 hours.

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External Sources

  1. View record in Web of Science®
  2. WOS: 000235832200016

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