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Detection of lymph node involvement in hematologic malignancies using micromagnetic resonance lymphangiography with a gadolinum-labeled dendrimer nanoparticle

  1. Author:
    Kobayashi, H.
    Kawamoto, S.
    Brechbiel, M. W.
    Bernardo, M.
    Sato, N.
    Waldmann, T. A.
    Tagaya, Y.
    Choyke, P. L.

  2. Author Address

    NCI, Mol Imaging Program, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21218 USA. NCI, Radiat Oncol Branch, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. NCI, Mol Imaging Program, Canc Res Ctr, SAIC Frederick,NIH, Frederick, MD 21701 USA. NCI, Metab Branch, Canc Res Ctr, NIH, Bethesda, MD 20892 USA.;Kobayashi, H, NCI, Mol Imaging Program, Canc Res Ctr, NIH, Bldg 10,Room 1 B40,MSC1088,10 Ctr Dr, Bethesda, MD 20892 USA.;kobayashi@mail.nih.gov
    1. Year: 2005
    2. Date: Nov
  2. Journal: Neoplasia
    1. 7
    2. 11
    3. Pages: 984-991
  4. Type of Article: Article
  5. ISSN: 1522-8002
  1. Abstract:

    Animal models of lymphoma should reflect their counterparts in humans; however, it can be difficult to ascertain whether an induced disease is intralymphatic or extralymphatic based on direct visualization. Current imaging methods are insufficient for identifying lymphatic and intralymphatic involvement. To differentiate intralymphatic from extralymphatic involvement, we have developed a magnetic resonance imaging-based lymphangiography method and tested it on two animal models of lymphoma. A gadolinium (Gd)-labeled dendrimer nanoparticle (generation-6; similar to 220 kDa/similar to 10 nm) was injected interstitially into mice bearing hematologic malignancies to perform dynamic micromagnetic resonance lymphangiography (micro-MRIL). Both a standard T1-weighted 3D fast spoiled gradient echo and a T2/T1-weighted D-fast imaging employing steady-state acquisition (3D-FIESTA-C) were compared in an imaging study to differentiate intralymphatic from extralymphatic involvement of tumors. The lymphatics and lymph nodes were visualized with both methods in all cases. In addition, 3D-FIESTA-C depicted both the lymphatic system and the extralymphatic tumor. In an animal model, 3D-FIESTA-C demonstrated that the bulk of the tumor thought to be intralymphatic was actually extralymphatic. In conclusion, micro-MRL, using Gd-labeled dendrimer nanoparticles with the combined method, can define both the normal and abnormal lymphatics and can distinguish intralymphatic from extralymphatic diseases in mouse models of malignant lymphoma.

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  2. DOI: 10.1593/neo.05454
  3. View record in Web of Science®
  4. WOS: 000234020800003

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