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R5 and X4 HIV envelopes induce distinct gene expression profiles in primary peripheral blood mononuclear cells

  1. Author:
    Cicala, C.
    Arthos, J.
    Martinelli, E.
    Censoplano, N.
    Cruz, C. C.
    Chung, E.
    Selig, S. M.
    Van Ryk, D.
    Yang, J.
    Jagannatha, S.
    Chun, T. W.
    Ren, P.
    Lempicki, R. A.
    Fauci, A. S.

  2. Author Address

    NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. Sci Applicat Int Corp, Lab Immunopathogenesis & Bioinformat, Frederick, MD 21702 USA.;Cicala, C, NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA.;ccicala@niaid.nih.gov afauci@niaid.nih.gov
    1. Year: 2006
    2. Date: Mar
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 103
    2. 10
    3. Pages: 3746-3751
  4. Type of Article: Article
  5. ISSN: 0027-8424
  1. Abstract:

    HIV envelope binds to and signals through its primary cellular receptor, CD4, and through a coreceptor, either CC chemokine receptor 5 (CCR5) or CXC chemokine receptor 4 (CXCR4). Here, we evaluate the response of peripheral blood mononuclear cells to a panel of genetically diverse R5 and X4 envelope proteins. Modulation of gene expression was evaluated by using oligonucleotide microarrays. Activation of transcription factors was evaluated by using an array of oligonucleotides encoding transcription factor binding sites. Responses were strongly influenced by coreceptor specificity. Treatment of cells from CCR5 Delta 32 homozygous donors with glycoprotein (gp)120 derived from an R5 virus demonstrated that the majority of responses elicited by R5 envelopes required engagement of CCR5. IRS envelopes, to a greater extent than X4 envelopes, induced the expression of genes belonging to mitogen-activated protein kinase signal transduction pathways and genes regulating the cell cycle. A number of genes induced by R5, but not X4, envelopes were also up-regulated in the resting CD4(+) T cell population of HIV-infected individuals. These results suggest that R5 envelope facilitates replication of HIV in the pool of resting CD4+ T cells. Additionally, signaling by R5 gp120 may facilitate the transmission of R5 viruses by inducing a permissive environment for HIV replication.

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External Sources

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  2. DOI: 10.1073/pnas.0511237103
  3. View record in Web of Science®
  4. WOS: 000236225300045

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