Skip NavigationSkip to Content
Return Return to Search Results

Influenza virus-assisted entry of a TCL-tropic HIV-1 isolate into CD4+ cells

  1. Author:
    Puri, A.
    Carroll, R.
    Riley, J.
    Hug, P.
    Jernigan, K.
    Blumenthal, R.
    1. Year of Conference: 1998
  2. Conference Name: Keystone Symposia on Molecular and Cellular Biology
    1. Pages: 114 (abstract no. 109)
  4. Type of Work: Meeting Abstract
  1. Abstract:

    We investigated the ability of human immunodeficiency virus type 1 (HIV-1) to infect CD4+ cells which were previously infected with an influenza virus strain (A/PR/8H1N1). Sub-confluent monolayers of cells were infected with influenza virus at 37 degrees C and subsequently tested for their susceptibility to HIV-1 infection by the detection of early and late transcripts in the virus life cycle at 0, 2 and 24 hours at 37 degrees C. CD4+ non-human cells (mink-CD4) became susceptible to HIV-1 entry when they were previously infected with influenza virus. Influenza virus-infected human CD4+ cells (primary human CD4+ and HeLa-CD4) showed significant enhancement of HIV-1 entry and replication. Influenza virus-mediated HIV-1 entry was only observed for a T-cell tropic HIV-1 (NL4-3). Entry of a macrophage-tropic HIV-1 strain (US1) was not influenced by prior infection of the target cells with influenza virus. This selectivity might be mediated by a significant increase in cell surface expression of TCL-tropic coreceptor CXCR4 in HeLa-CD4 cells, as shown by immunofluorescence. Our observations may be significant in understanding the mechanisms of a shift from M-tropic to TCL-tropic HIV-1 phenotype associated with occurrence of opportunistic infections in HIV-1 infected individuals.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel