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Structural basis of interaction between urokinase-type plasminogen activator and its receptor

  1. Author:
    Barinka, C.
    Parry, G.
    Callahan, J.
    Shaw, D. E.
    Kuo, A.
    Bdeir, K.
    Cines, D. B.
    Mazar, A.
    Lubkowski, J.

  2. Author Address

    NCI, Canc Res Ctr, Frederick, MD 21702 USA. Attenuon, LLC, San Diego, CA 92121 USA. DE Shaw Res & Dev, New York, NY 10036 USA. Univ Penn, Med Ctr, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA.;Lubkowski, J, NCI, Canc Res Ctr, Frederick, MD 21702 USA.;jacek@ncifcrf.gov
    1. Year: 2006
    2. Date: Oct
  2. Journal: Journal of Molecular Biology
    1. 363
    2. 2
    3. Pages: 482-495
  4. Type of Article: Article
  5. ISSN: 0022-2836
  1. Abstract:

    Recent studies indicate that binding of the urokinase-type plasminogen activator (uPA) to its high-affinity receptor (uPAR) orchestrates uPAR interactions with other cellular components that play a pivotal role in diverse (patho-)physiological processes, including wound healing, angiogenesis, inflammation, and cancer metastasis. However, notwithstanding the wealth of biochemical data available describing the activities of uPAR, little is known about the exact mode of uPAR/uPA interactions or the presumed conformational changes that accompany uPA/uPAR engagement. Here, we report the crystal structure of soluble urokinase plasminogen activator receptor (suPAR), which contains the three domains of the wild-type receptor but lacks the cell-surface anchoring sequence, in complex with the amino-terminal fragment of urokinase-type plasminogen activator (ATF), at the resolution of 2.8 angstrom. We report the 1.9 angstrom crystal structure of free ATF. Our results provide a structural basis, represented by conformational changes induced in uPAR, for several published biochemical observations describing the nature of uPAR/uPA interactions and provide insight into mechanisms that may be responsible for the cellular responses induced by uPA binding. (c) 2006 Elsevier Ltd. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.jmb.2006.08.063
  3. View record in Web of Science®
  4. WOS: 000241324500015

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