Skip NavigationSkip to Content
Return Return to Search Results

Regulation of AID expression in the immune response

  1. Author:
    Crouch, E. E.
    Li, Z. Y.
    Takizawa, M.
    Fichtner-Feigl, S.
    Gourzi, P.
    Montano, C.
    Feigenbaum, L.
    Wilson, P.
    Janz, S.
    Papavasiliou, F. N.
    Casellas, R.

  2. Author Address

    NCI, Genom Integr & Immun, NIAMS, NIH, Bethesda, MD 20892 USA. NCI, NIAID, Mucosal Immun Sect, NIH, Bethesda, MD 20892 USA. NCI, Lab Genet, NIH, Bethesda, MD 20892 USA. Rockefeller Univ, Lab Lymphocyte Biol, New York, NY 10021 USA. NCI, Lab Anim Sci Program, Sci Applicat Int Corp, SAIC,NIH, Ft Detrick, MD 21702 USA. Oklahoma Med Res Fdn, Mol Immunogenet, Oklahoma City, OK 73104 USA.;Casellas, R, NCI, Genom Integr & Immun, NIAMS, NIH, Bethesda, MD 20892 USA.;casellar@mail.nih.gov
    1. Year: 2007
    2. Date: May
  2. Journal: Journal of Experimental Medicine
    1. 204
    2. 5
    3. Pages: 1145-1156
  4. Type of Article: Article
  5. ISSN: 0022-1007
  1. Abstract:

    The B cell-specific enzyme activation-induced cytidine deaminase (AID) has been shown to be essential for isotype switching and affinity maturation of antibody genes during the immune response. Conversely, AID activity has also been linked to autoimmunity and tumorigenesis. Determining how AID expression is regulated in vivo is therefore central to understanding its role in health and disease. Here we use phylogenetic footprinting and high-resolution histone acetylation mapping to accurately demarcate AID gene regulatory boundaries. Based on this strategy, we identify a novel, positive regulatory element required for AID transcription. Furthermore, we generate two AID indicator mouse strains using bacterial artificial chromosomes that faithfully recapitulate endogenous AID expression. The first strain uses a green fluorescent protein reporter to identify B cells that actively express AID during the immune response. In the second strain, AID transcription affects the permanent expression of a yellow fluorescent protein reporter in post-germinal center and terminally differentiated lymphocytes. We demonstrate the usefulness of these novel strains by resolving recent contradictory observations on AID expression during B cell ontogeny.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1084/jem.20061952
  3. View record in Web of Science®
  4. WOS: 000246467600018

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel