Skip NavigationSkip to Content
Return Return to Search Results

The role of electrophoresis in disease biomarker discovery

  1. Author:
    Issaq, H. J.
    Veenstra, T. D.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick Inc, Lab Proteom & Analyt Technologoies, Frederick, MD 21702 USA.;Issaq, HJ, NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick Inc, Lab Proteom & Analyt Technologoies, Frederick, MD 21702 USA.;issaqh@mail.ncifcrf.gov
    1. Year: 2007
    2. Date: Jun
  2. Journal: Electrophoresis
    1. 28
    2. 12
    3. Pages: 1980-1988
  4. Type of Article: Review
  5. ISSN: 0173-0835
  1. Abstract:

    There has been increased activity in the last few years in the search for disease markers using fractionation of complex biological fluids combined with MS. While electrophoretic and chromatographic separations have played a major role in this endeavor, this manuscript is limited to a review of electrophoretic methods that have been established for disease biomarker discovery. These methods include 2-DE, difference gel electrophoresis (DIGE), and CE. We define what constitutes a biomarker, identify the steps required for establishing a biomarker, and describe the parameters needed in the design of an ideal diagnostic test. The application, advantages, and limitations of CE, DIGE, and 2-DE in meeting the goal of discovering novel biomarkers is discussed in detail, along with a few selected examples that illustrate the search for biomarkers for cancer and neurological diseases.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1002/elps.200600834
  3. View record in Web of Science®
  4. WOS: 000247936000018

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel