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Detection of KIR3DS1 on the cell surface of peripheral blood NK cells facilitates identification of a novel null allele and assessment of KIR3DS1 expression during HIV-1 infection

  1. Author:
    Pascal, V.
    Yamada, E.
    Martin, M. P.
    Alter, G.
    Altfeld, M.
    Metcalf, J. A.
    Baseler, M. W.
    Adelsberger, J. W.
    Carrington, M.
    Anderson, S. K.
    McVicar, D. W.

  2. Author Address

    NCI, Ctr Canc Res, Expt Immunol Lab, Canc Inflammat Program, Frederick, MD 21702 USA. NCI, SAIC Frederick Inc, Basic Res Program, Frederick, MD 21702 USA. Harvard Univ, Sch Med, Massachusetts Gen Hosp, Infect Dis Unit,Partners AIDS Res Ctr, Boston, MA 02115 USA. Harvard Univ, Sch Med, Div Aids, Boston, MA 02115 USA. Natl Inst Hlth, NIAID, Div Clin Res, Bethesda, MD 20892 USA. NCI, SAIC Frederick, Clin Serv Program, AIDS Monitoring Lab, Frederick, MD 21702 USA.;McVicar, DW, NCI, Ctr Canc Res, Expt Immunol Lab, Canc Inflammat Program, Bldg 560 Room 31-46, Frederick, MD 21702 USA.;mcvicar@nih.gov
    1. Year: 2007
    2. Date: Aug
  2. Journal: Journal of Immunology
    1. 179
    2. 3
    3. Pages: 1625-1633
  4. Type of Article: Article
  5. ISSN: 0022-1767
  1. Abstract:

    KIR3DL1 is a highly polymorphic killer cell Ig-like receptor gene with at least 23 alleles described, including its activating counterpart, KIR3DS1. Recently, the KIR3DS1 allele has been shown to slow progression to AIDS in individuals expressing HLA-Bw4 with isoleucine at position 80. However, due to the lack of a specific All, KIR3DS1 expression and function is not well characterized. In this study, we demonstrate KIR3DS1 expression on a substantial subset of peripheral natural killer cells through its recognition by the mAb Z27. The fidelity of this detection method was confirmed by analysis of KIR3DS1 transfectants and the identification of a novel KIR3DS1 null allele. Interestingly, KIR3DS1 is also expressed by a small proportion of CD56(+) T cells. We show that ligation of KIR3DS1 by Z27 leads to NK cell IFN-gamma production and degranulation as assessed by expression of CD107a. Furthermore, we document the persistence of KIR3DS1(+) NK cells in HIV-1 viremic patients. The high frequency of KIR3DS1 expression, along with its ability to activate NK cells, and its maintenance during HIV-1 viremia are consistent with the epidemiological data suggesting a critical role for this receptor in controlling HIV-1 pathogenesis.

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External Sources

  1. View record in Web of Science®
  2. WOS: 000248319700026

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