Skip NavigationSkip to Content
Return Return to Search Results

A method of HIV-1 inactivation compatible with antibody-based depletion of abundant proteins from plasma

  1. Author:
    Bosley, A.
    Marshall, H. N.
    Badralmaa, Y.
    Natarajan, V.

  2. Author Address

    Bosley, Allen, Marshall, Heather N.; Badralmaa, Yunden, Natarajan, Ven] NCI, Mol Cell Biol Lab, SAIC Frederick, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Proteomics Clinical Applications
    1. 2
    2. 6
    3. Pages: 904-907
  4. Type of Article: Article
  1. Abstract:

    We have examined several methods, including heat treatment and treatment with detergents, to inactivate HIV-1 present in plasma to be depleted of abundant proteins utilizing an antibody-based technology. Treatment with Triton X-100 was not compatible with abundant protein depletion with an antibody column and heat treatment alters the composition of the plasma proteome. However, treatment with 1.2% N-octylglucoside for 5 min completely inhibited HIV-1 infectivity. The detergent was easily removed through buffer exchange, and this treatment had no discernable effect on protein depletion.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel