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Structural and Functional Analysis of the E. coli NusB-S10 Transcription Antitermination Complex

  1. Author:
    Luo, X.
    Hsiao, H. H.
    Bubunenko, M.
    Weber, G.
    Court, D. L.
    Gottesman, M. E.
    Urlaub, H.
    Wahl, M. C.

  2. Author Address

    Gottesman, Max E.] Columbia Univ, Med Ctr, Dept Microbiol & Biochem, New York, NY 10032 USA. [Gottesman, Max E.] Columbia Univ, Med Ctr, Dept Mol Biophys, New York, NY 10032 USA. [Luo, Xiao, Weber, Gert, Wahl, Markus C.] Max Planck Inst Biophys Chem, Res Grp Xray Crystallog, D-37077 Gottingen, Germany. [Hsiao, He-Hsuan, Urlaub, Henning] Max Planck Inst Biophys Chem, Res Grp Bioanalyt Mass Spectrometry, D-37077 Gottingen, Germany. [Bubunenko, Mikhail, Court, Donald L.] NCI, Gene Regulat & Chromosomal Biol Lab, Ctr Canc Res, Frederick, MD 21702 USA. [Bubunenko, Mikhail] NCI, Basic Res Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Wahl, Markus C.] Univ Gottingen, Dept Med, D-37077 Gottingen, Germany.
    1. Year: 2008
  2. Journal: Molecular Cell
    1. 32
    2. 6
    3. Pages: 791-802
  4. Type of Article: Article
  1. Abstract:

    Protein S10 is a component of the 30S ribosomal subunit and participates together with NusB protein in processive transcription antitermination. The molecular mechanisms by which S10 can act as a translation or a transcription factor are not understood. We used complementation assays and recombineering to delineate regions of S10 dispensable for antitermination, and determined the crystal structure of a transcriptionally active NusB-S10 complex. In this complex, S10 adopts the same fold as in the 30S subunit and is blocked from simultaneous association with the ribosome. Mass spectrometric mapping of UV-induced crosslinks revealed that the NusB-S10 complex presents an intermolecular, composite, and contiguous binding surface for RNAs containing BoxA antitermination signals. Furthermore, S10 overproduction complemented a nusB null phenotype. These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination.

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External Sources

  1. PMID: 19111659

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