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Sizing the Bacillus anthracis PA(63) channel with nonelectrolyte poly(ethylene glycols)

  1. Author:
    Nablo, B. J.
    Halverson, K. M.
    Robertson, J.
    Nguyen, T. L.
    Panchal, R. G.
    Gussio, R.
    Bavari, S.
    Krasilnikov, O. V.
    Kasianowicz, J. J.

  2. Author Address

    Nablo, Brian J.; Robertson, Joseph W. F.; Kasianowicz, John J.] NIST, Div Semicond Elect, Elect & Elect Lab, Gaithersburg, MD 20899 USA. [Halverson, Kelly M.] USA, Ctr Environm Hlth Res, Frederick, MD USA. [Nguyen, Tam L.; Panchal, Rekha G.; Gussio, Rick] NCI, Target Struct Based Drug Discovery Grp, SAIC Frederick, Frederick, MD 21701 USA. [Bavari, Sina] USA, Med Res Inst Infect Dis, Frederick, MD USA. [Krasilnikov, Oleg V.] Univ Fed Pernambuco, Lab Membrane Biophys, Dept Biophys & Radiobiol, Recife, PE, Brazil.
    1. Year: 2008
  2. Journal: Biophysical Journal
    1. 95
    2. 3
    3. Pages: 1157-1164
  4. Type of Article: Article
  1. Abstract:

    Nonelectrolyte polymers of poly(ethylene glycol) (PEG) were used to estimate the diameter of the ion channel formed by the Bacillus anthracis protective antigen 63 (PA(63)). Based on the ability of different molecular weight PEGs to partition into the pore and reduce channel conductance, the pore appears to be narrower than the one formed by Staphylococcus aureus a-hemolysin. Numerical integration of the PEG sample mass spectra and the channel conductance data were used to refine the estimate of the pore's PEG molecular mass cutoff (similar to 1400 g/mol). The results suggest that the limiting diameter of the PA(63) pore is < 2 nm, which is consistent with an all-atom model of the PA(63) channel and previous experiments using large ions.

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External Sources

  1. PMID: 18645196

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