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Ceramide kinase regulates phospholipase C and phosphatidylinositol 4, 5, bisphosphate in phototransduction

  1. Author:
    Dasgupta, U.
    Bamba, T.
    Chiantia, S.
    Karim, P.
    Abou Tayoun, A. N.
    Yonamine, I.
    Rawat, S. S.
    Rao, R. P.
    Nagashima, K.
    Fukusaki, E.
    Puri, V.
    Dolph, P. J.
    Schwille, P.
    Acharya, J. K.
    Acharya, U.

  2. Author Address

    Dasgupta, Ujjaini, Karim, Pusha, Yonamine, Ikuko, Rawat, Satinder S.; Acharya, Usha] Univ Massachusetts, Sch Med, Program Gene Funct & Express, Worcester, MA 01605 USA. [Dasgupta, Ujjaini, Karim, Pusha, Yonamine, Ikuko, Rawat, Satinder S.; Puri, Vishwajeet, Acharya, Usha] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA. [Bamba, Takeshi, Fukusaki, Eiichiro] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan. [Chiantia, Salvatore, Schwille, Petra] Tech Univ Dresden, Biotechnol Zentrum, D-01187 Dresden, Germany. [Abou Tayoun, Ahmad N.; Dolph, Patrick J.] Dartmouth Coll, Dept Biol, Hanover, NH 03755 USA. [Rao, Raghavendra Pralhada, Acharya, Jairaj K.] NCI, Lab Cell & Dev Signaling, Frederick, MD 21702 USA. [Nagashima, Kunio] SAIC, EM Facil, Image Anal Lab, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 106
    2. 47
    3. Pages: 20063-20068
  4. Type of Article: Article
  5. ISSN: 0027-8424
  1. Abstract:

    Phosphoinositide-specific phospholipase C (PLC) is a central effector for many biological responses regulated by G-protein-coupled receptors including Drosophila phototransduction where light sensitive channels are activated downstream of NORPA, a PLC beta homolog. Here we show that the sphingolipid biosynthetic enzyme, ceramide kinase, is a novel regulator of PLC signaling and photoreceptor homeostasis. A mutation in ceramide kinase specifically leads to proteolysis of NORPA, consequent loss of PLC activity, and failure in light signal transduction. The mutant photoreceptors also undergo activity-dependent degeneration. Furthermore, we show that a significant increase in ceramide, resulting from lack of ceramide kinase, perturbs the membrane microenvironment of phosphatidylinositol 4, 5, bisphosphate (PIP2), altering its distribution. Fluorescence image correlation spectroscopic studies on model membranes suggest that an increase in ceramide decreases clustering of PIP2 and its partitioning into ordered membrane domains. Thus ceramide kinase-mediated maintenance of ceramide level is important for the local regulation of PIP2 and PLC during phototransduction.

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  2. DOI: 10.1073/pnas.0911028106
  3. PMID: 19892737

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