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The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription

  1. Author:
    Rochman, M.
    Postnikov, Y.
    Correll, S.
    Malicet, C.
    Wincovitch, S.
    Karpova, T. S.
    McNally, J. G.
    Wu, X. L.
    Bubunenko, N. A.
    Grigoryev, S.
    Bustin, M.

  2. Author Address

    Rochman, Mark, Postnikov, Yuri, Malicet, Cedric, Bustin, Michael] NCI, Prot Sect, Lab Metab, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. [Wincovitch, Stephen] NHGRI, Genet Dis Res Branch, NIH, Bethesda, MD 20892 USA. [Karpova, Tatiana S.; McNally, James G.] NCI, CCR Core Fluorescence Imaging Facil, Lab Receptor Biol & Gene Express, NIH, Bethesda, MD 20892 USA. [Correll, Sarah, Grigoryev, Sergei] Penn State Univ, Coll Med, Dept Biochem & Mol Biol, Hershey, PA 17033 USA. [Wu, Xiaolin, Bubunenko, Nina A.] NCI, Lab Mol Technol, SAIC Frederick, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Molecular Cell
    1. 35
    2. 5
    3. Pages: 642-656
  4. Type of Article: Article
  1. Abstract:

    Structural changes in specific chromatin domains are essential to the orderly progression of numerous nuclear processes, including transcription. We report that the nuclear protein NSBP1 (HMGN5), a recently discovered member of the HMGN nucleosome-binding protein family, is specifically targeted by its C-terminal domain to nucleosomes in euchromatin. We find that the interaction of NSBP1 with nucleosomes alters the compaction of cellular chromatin and that in living cells, NSBP1 interacts with linker histones. We demonstrate that the negatively charged C-terminal domain of NSBP1 interacts with the positively charged C-terminal domain of H5 and that NSBP1 counteracts the linker histone-mediated compaction of a nucleosomal array. Dysregulation of the cellular levels of NSBP1 alters the transcription level of numerous genes. We suggest that mouse NSBP1 is an architectural protein that binds preferentially to euchromatin and modulates the fidelity of the cellular transcription profile by counteracting the chromatin-condensing activity of linker histories.

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  2. DOI: 10.1016/j.molcel.2009.07.002
  3. PMID: 19748358

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