Skip NavigationSkip to Content
Return Return to Search Results

Rapamycin Protects Mice from Staphylococcal Enterotoxin B-Induced Toxic Shock and Blocks Cytokine Release In Vitro and In Vivo

  1. Author:
    Krakauer, T.
    Buckley, M.
    Issaq, H. J.
    Fox, S. D.

  2. Author Address

    [Krakauer, Teresa; Buckley, Marilyn] USA, Med Res Inst Infect Dis, Dept Immunol, Integrated Toxicol Div, Frederick, MD 21702 USA. [Issaq, Haleem J.; Fox, Stephen D.] Sci Applicat Int Corp Frederick Inc, Lab Prote & Analyt Technol, Frederick, MD 21702 USA.;Krakauer, T, USA, Med Res Inst Infect Dis, Dept Immunol, Integrated Toxicol Div, Bldg 1425, Frederick, MD 21702 USA.;teresa.krakauer@amedd.army.mil
    1. Year: 2010
    2. Date: Mar
  2. Journal: Antimicrobial Agents and Chemotherapy
    1. 54
    2. 3
    3. Pages: 1125-1131
  4. Type of Article: Article
  5. ISSN: 0066-4804
  1. Abstract:

    Staphylococcal enterotoxins are potent activators for human T cells and cause lethal toxic shock. Rapamycin, an immunosuppressant, was tested for its ability to inhibit staphylococcal enterotoxin B (SEB)-induced activation of human peripheral blood mononuclear cells (PBMC) in vitro and toxin-mediated shock in mice. Stimulation of PMBC by SEB was effectively blocked by rapamycin as evidenced by the inhibition of tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), IL-6, IL-2, gamma interferon (IFN-gamma), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1 alpha (MIP-1 alpha), MIP-1 beta, and T-cell proliferation. In vivo, rapamycin protected 100% of mice from lethal shock, even when administered 24 h after intranasal SEB challenge. The serum levels of MCP-1 and IL-6, after intranasal exposure to SEB, were significantly reduced in mice given rapamycin versus controls. Additionally, rapamycin diminished the weight loss and temperature fluctuations elicited by SEB.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1128/aac.01015-09
  3. View record in Web of Science®
  4. WOS: 000274733300021

Library Notes

  1. Fiscal Year: FY2009-2010
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel