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Preparation and characterization of a magnetic and optical dual-modality molecular probe

  1. Author:
    Bumb, A.
    Regino, C. A. S.
    Perkins, M. R.
    Bernardo, M.
    Ogawa, M.
    Fugger, L.
    Choyke, P. L.
    Dobson, P. J.
    Brechbiel, M. W.

  2. Author Address

    [Bumb, A.; Brechbiel, M. W.] NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA. [Regino, C. A. S.; Ogawa, M.; Choyke, P. L.] NCI, Mol Imaging Program, NIH, Bethesda, MD 20892 USA. [Perkins, M. R.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. [Perkins, M. R.; Fugger, L.] Univ Oxford, John Radcliffe Hosp, MRC Human Immunol Unit, Weatherall Inst Mol Med, Oxford OX3 9DS, England. [Bernardo, M.] NCI Frederick, SAIC Frederick Inc, Frederick, MD 21702 USA. [Dobson, P. J.] Univ Oxford, Kidlington OX5 1PF, Oxon, England.;Bumb, A, NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, NIH, Bldg 10,Room 1B53,10 Ctr Dr, Bethesda, MD 20892 USA.;Bumba@mail.nih.gov martinwb@mail.nih.gov
    1. Year: 2010
    2. Date: Apr
  2. Journal: Nanotechnology
    1. 21
    2. 17
    3. Pages: 9
  4. Type of Article: Article
  5. Article Number: 175704
  6. ISSN: 0957-4484
  1. Abstract:

    Multi-modality imaging probes combine the advantages of individual imaging techniques to yield highly detailed anatomic and molecular information in living organisms. Herein, we report the synthesis and characterization of a dual-modality nanoprobe that couples the magnetic properties of ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) with the near infrared fluorescence of Cy5.5. The fluorophore is encapsulated in a biocompatible shell of silica surrounding the iron oxide core for a final diameter of similar to 17 nm. This silica-coated iron oxide nanoparticle (SCION) has been analyzed by transmission electron microscopy, dynamic light scattering, and superconducting quantum interference device (SQUID). The particle demonstrates a strong negative surface charge and maintains colloidal stability in the physiological pH range. Magnetic hysteresis analysis confirms superparamagnetic properties that could be manipulated for thermotherapy. The viability of primary human monocytes, T cells, and B cells incubated with the particle has been examined in vitro. In vivo analysis of agent leakage into subcutaneous A431 tumors in mice was also conducted. This particle has been designed for diagnostic application with magnetic resonance and fluorescence imaging, and has future potential to serve as a heat-sensitive targeted drug delivery platform.

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External Sources

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  2. DOI: 10.1088/0957-4484/21/17/175704
  3. View record in Web of Science®
  4. WOS: 000276671300019

Library Notes

  1. Fiscal Year: FY2009-2010
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