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Long-lasting humoral and cellular immune responses and mucosal dissemination after intramuscular DNA immunization

  1. Author:
    Patel, V.
    Valentin, A.
    Kulkarni, V.
    Rosati, M.
    Bergamaschi, C.
    Jalah, R.
    Alicea, C.
    Minang, J. T.
    Trivett, M. T.
    Ohlen, C.
    Zhao, J.
    Robert-Guroff, M.
    Khan, A. S.
    Draghia-Akli, R.
    Felber, B. K.
    Pavlakis, G. N.

  2. Author Address

    [Jalah, Rashmi; Alicea, Candido; Felber, Barbara K.] NCI, Human Retrovirus Pathogenesis Sect, Vaccine Branch, Ctr Canc Res, Frederick, MD 21702 USA. [Patel, Vainav; Valentin, Antonio; Rosati, Margherita; Bergamaschi, Cristina; Pavlakis, George N.] NCI, Human Retrovirus Sect, Vaccine Branch, Ctr Canc Res, Frederick, MD 21702 USA. [Minang, Jacob T.; Trivett, Matthew T.; Ohlen, Claes] NCI, AIDS & Canc Virus Program, SAIC, Frederick, MD 21702 USA. [Zhao, Jun; Robert-Guroff, Marjorie] NCI, Immune Biol Retroviral Infect Sect, Vaccine Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Khan, Amir S.; Draghia-Akli, Ruxandra] Inovio Biomed Corp, Blue Bell, PA 19422 USA.;Felber, BK, NCI, Human Retrovirus Pathogenesis Sect, Vaccine Branch, Ctr Canc Res, POB B,Bldg 535,Room 206, Frederick, MD 21702 USA.;felberb@mail.nih.gov pavlakig@mail.nih.gov
    1. Year: 2010
    2. Date: Jul
  2. Journal: Vaccine
    1. 28
    2. 30
    3. Pages: 4827-4836
  4. Type of Article: Article
  5. ISSN: 0264-410X
  1. Abstract:

    Naive Indian rhesus macaques were immunized with a mixture of optimized plasmid DNAs expressing several Sly antigens using in vivo electroporation via the intramuscular route. The animals were monitored for the development of Sly-specific systemic (blood) and mucosal (bronchoalveolar lavage) cellular and humoral immune responses. The immune responses were of great magnitude, broad (Gag, Pol, Nef, Tat and Vif), long-lasting (up to 90 weeks post third vaccination) and were boosted with each subsequent immunization, even after an extended 90-week rest period. The Sly-specific cellular immune responses were consistently more abundant in bronchoalveolar lavage (BAL) than in blood, and were characterized as predominantly effector memory CD4(+) and CDS+ T cells in BAL and as both central and effector memory T cells in blood. Sly-specific T cells containing Granzyme B were readily detected in both blood and BAL, suggesting the presence of effector cells with cytolytic potential. DNA vaccination also elicited long-lasting systemic and mucosal humoral immune responses, including the induction of Gag-specific IgA. The combination of optimized DNA vectors and improved intramuscular delivery by in vivo electroporation has the potential to elicit both cellular and humoral responses and dissemination to the periphery, and thus to improve DNA immunization efficacy. Published by Elsevier Ltd.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.vaccine.2010.04.064
  3. View record in Web of Science®
  4. WOS: 000280345700021

Library Notes

  1. Fiscal Year: FY2009-2010
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