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Insulin enhances the effect of nitric oxide at inhibiting neointimal hyperplasia in a rat model of type 1 diabetes

  1. Author:
    Varu, V. N.
    Ahanchi, S. S.
    Hogg, M. E.
    Bhikhapurwala, H. A.
    Chen, A.
    Popowich, D. A.
    Vavra, A. K.
    Martinez, J.
    Jiang, Q.
    Saavedra, J. E.
    Hrabie, J. A.
    Keefer, L. K.
    Kibbe, M. R.

  2. Author Address

    [Varu, Vinit N.; Ahanchi, Sadie S.; Hogg, Melissa E.; Bhikhapurwala, Hussein A.; Chen, Amy; Popowich, Daniel A.; Vavra, Ashley K.; Martinez, Janet; Jiang, Qun; Kibbe, Melina R.] Northwestern Univ, Div Vasc Surg, Chicago, IL 60611 USA. [Varu, Vinit N.; Ahanchi, Sadie S.; Hogg, Melissa E.; Bhikhapurwala, Hussein A.; Chen, Amy; Popowich, Daniel A.; Vavra, Ashley K.; Martinez, Janet; Jiang, Qun; Kibbe, Melina R.] Northwestern Univ, Inst BioNanotechnol Med, Chicago, IL 60611 USA. [Varu, Vinit N.] Northwestern Univ, Dept Surg, Chicago, IL 60611 USA. [Ahanchi, Sadie S.] Univ Illinois Mt Sinai, Dept Surg, Chicago, IL USA. [Saavedra, Joseph E.; Hrabie, Joseph A.] SAIC Frederick Inc, Basic Res Program, Frederick, MD USA. [Keefer, Larry K.] Natl Canc Inst Frederick, Comparat Carcinogenesis Lab, Ctr Canc Res, Frederick, MD USA. [Kibbe, Melina R.] Jesse Brown Vet Affairs Med Ctr, Chicago, IL USA.;Kibbe, MR, Northwestern Univ, Div Vasc Surg, 676 N St Clair St 650, Chicago, IL 60611 USA.;mkibbe@nmh.org
    1. Year: 2010
    2. Date: Sep
  2. Journal: American Journal of Physiology-Heart and Circulatory Physiology
    1. 299
    2. 3
    3. Pages: H772-H779
  4. Type of Article: Article
  5. ISSN: 0363-6135
  1. Abstract:

    Varu VN, Ahanchi SS, Hogg ME, Bhikhapurwala HA, Chen A, Popowich DA, Vavra AK, Martinez J, Jiang Q, Saavedra JE, Hrabie JA, Keefer LK, Kibbe MR. Insulin enhances the effect of nitric oxide at inhibiting neointimal hyperplasia in a rat model of type 1 diabetes. Am J Physiol Heart Circ Physiol 299: H772-H779, 2010. First published June 18, 2010; doi:10.1152/ajpheart.01234.2009.-Diabetes confers greater restenosis from neointimal hyperplasia following vascular interventions. While localized administration of nitric oxide (NO) is known to inhibit neointimal hyperplasia, the effect of NO in type 1 diabetes is unknown. Thus the aim of this study was to determine the efficacy of NO following arterial injury, with and without exogenous insulin administration. Vascular smooth muscle cells (VSMC) from lean Zucker (LZ) rats were exposed to the NO donor, DETA/NO, following treatment with different glucose and/or insulin concentrations. DETA/NO inhibited VSMC proliferation in a concentration-dependent manner to a greater extent in VSMC exposed to normal-glucose vs. high-glucose environments, and even more effectively in normal-glucose/high-insulin and high-glucose/high-insulin environments. G(0)/G(1) cell cycle arrest and cell death were not responsible for the enhanced efficacy of NO in these environments. Next, type 1 diabetes was induced in LZ rats with streptozotocin. The rat carotid artery injury model was performed. Type 1 diabetic rats experienced no significant reduction in neointimal hyperplasia following arterial injury and treatment with the NO donor PROLI/NO. However, daily administration of insulin to type 1 diabetic rats restored the efficacy of NO at inhibiting neointimal hyperplasia (60% reduction, P < 0.05). In conclusion, these data demonstrate that NO is ineffective at inhibiting neointimal hyperplasia in an uncontrolled rat model of type 1 diabetes; however, insulin administration restores the efficacy of NO at inhibiting neointimal hyperplasia. Thus insulin may play a role in regulating the downstream beneficial effects of NO in the vasculature.

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External Sources

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  2. DOI: 10.1152/ajpheart.01234.2009
  3. View record in Web of Science®
  4. WOS: 000281537400023

Library Notes

  1. Fiscal Year: FY2009-2010
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