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Cloning and characterization of a novel activating Ly49 closely related to Ly49A

  1. Author:
    Makrigiannis, A. P.
    Gosselin, P.
    Mason, L. H.
    Taylor, L. S.
    McVicar, D. W.
    Ortaldo, J. R.
    Anderson, S. K.

  2. Author Address

    Anderson SK NCI, Intramural Res Support Program, Sci Applicat Int Corp, Frederick Canc Res & Dev Ctr Bldg 560,Room 31-93 Frederick, MD 21702 USA NCI, Intramural Res Support Program, Sci Applicat Int Corp, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, Expt Immunol Lab, Div Basic Sci, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 1999
  2. Journal: Journal of Immunology
    1. 163
    2. 9
    3. Pages: 4931-4938
  4. Type of Article: Article
  1. Abstract:

    The majority of the known Ly49 family members have been isolated from either C57BL/6 (B6) or BALB/c mice, Interestingly, the anti-Ly49 Ab reactivities observed in 129/J mice are different from those of B6 mice. Furthermore, immunoprecipitation of 129/J NK cell lysates with YE1/32 and YE1/48, Abs specific for the inhibitory Ly49A in B6, resulted in detection of the activation-associated DAP12 molecule. These results indicated a need for a more detailed study of this strain. Therefore, cloning strategy was devised to isolate Ly49 cDNAs from 129/J mice. An immunoreceptor tyrosine-based inhibitory motif-containing, Ly49D-related clone was discovered that we have named Ly49O, and one immunoreceptor tyrosine-based inhibitory motif-lacking, Ly49A-related clone was discovered that we have named Ly49P. No anti-Ly49 mAb reacted with Ly49O, whereas the molecule encoded by the Ly49P cDNA was found to react with YE1/32 and YE1/48. Ly49P was found to associate with mouse DAP12, and Ah-mediated cross-linking of Ly49P resulted in mouse DAP12 phosphorylation and Ca2+ mobilization, indicating that Ly49P is a competent activation receptor. Ly49P, therefore, represents a novel member of the Ly49 activating receptor subfamily. [References: 21]

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