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Defining functional domains of Ku80: DNA end binding and survival after radiation

  1. Author:
    Osipovich, O.
    Duhe, R. J.
    Hasty, P.
    Durum, S. K.
    Muegge, K.

  2. Author Address

    Osipovich O NCI, Mol Immunoregulat Lab, SAIC Frederick, MD 21702 USA NCI, Mol Immunoregulat Lab, SAIC Frederick, MD 21702 USA Lexicon Genet Inc The Woodlands, TX 77382 USA
    1. Year: 1999
  2. Journal: Biochemical and Biophysical Research Communications
    1. 261
    2. 3
    3. Pages: 802-807
  4. Type of Article: Article
  1. Abstract:

    The Ku heterodimeric protein (Ku80/Ku70) is an essential component of the double-strand break DNA repair pathway in mammalian cells, We have recently defined a central region within Ku80 that is required for heterodimerization with Ku70, We now identified a core region within Ku80 (amino acids 210 to 531) that is necessary for binding of Ku to DNA ends, Interaction with Ku70 and DNA end binding are important for Ru80 function in vivo since Ku80 mutants lacking DNA end binding activity were unable to restore radiation resistance in Ku80 deficient fibroblast cell lines. However, Ku80 mutants were identified that retained DNA end binding activity but were unable to restore radiation survival, thus pointing to additional functional properties of Ku80. An N-terminal deletional mutant of Ku80 was able to suppress wild type Ku80 function for radiation survival in several cell lines, thus demonstrating dominant negative function. (C) 1999 Academic Press. [References: 16]

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