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The mechanism of RNA duplex recognition and unwinding by DEAD-box helicase DDX3X

  1. Author:
    Song, He
    Ji,Xinhua

  2. Author Address

    NCI, Macromol Crystallog Lab, Frederick, MD 21702 USA.
    1. Year: 2019
    2. Date: Jul 12
    3. Epub Date: 2019 07 12
  2. Journal: NATURE COMMUNICATIONS
  3. NATURE PUBLISHING GROUP,
    1. 10
    2. 1
  5. Type of Article: Article
  6. Article Number: 3085
  7. ISSN: 2041-1723
  1. Abstract:

    DEAD-box helicases (DDXs) regulate RNA processing and metabolism by unwinding short double-stranded (ds) RNAs. Sharing a helicase core composed of two RecA-like domains (D1D2), DDXs function in an ATP-dependent, non-processive manner. As an attractive target for cancer and AIDS treatment, DDX3X and its orthologs are extensively studied, yielding a wealth of biochemical and biophysical data, including structures of apo-D1D2 and post-unwound D1D2: single-stranded RNA complex, and the structure of a D2: dsRNA complex that is thought to represent a pre-unwound state. However, the structure of a pre-unwound D1D2: dsRNA complex remains elusive, and thus, the mechanism of DDX action is not fully understood. Here, we describe the structure of a D1D2 core in complex with a 23-base pair dsRNA at pre-unwound state, revealing that two DDXs recognize a 2-turn dsRNA, each DDX mainly recognizes a single RNA strand, and conformational changes induced by ATP binding unwinds the RNA duplex in a cooperative manner.

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  1. Keywords:

External Sources

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  2. DOI: 10.1038/s41467-019-11083-2
  3. PMID: 31300642
  4. View record in Web of Science®
  5. WOS: 000475295300007

Library Notes

  1. Fiscal Year: FY2018-2019
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