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Expansion microscopy for the analysis of centrioles and cilia

  1. Author:
    Sahabandu, N
    Kong,Dong
    Magidson,Valentin
    Nanjundappa, R
    Sullenberger,Catherine
    Mahjoub, M
    Loncarek,Jadranka [ORCID]

  2. Author Address

    Laboratory of Protein Dynamics and Signaling, NIH/NCI/CCR, Frederick, Maryland, 21702, USA., Optical Microscopy and Analysis Laboratory, Frederick National Laboratory for Cancer Research, Frederick, Maryland, 21702, USA., Department of Medicine (Nephrology Division), Washington University, St Louis, MO, USA.,
    1. Year: 2019
    2. Date: Nov 19
    3. Epub Date: 2019 11 06
  2. Journal: Journal of microscopy
  4. Type of Article: Article
  5. ISSN: 0022-2720
  1. Abstract:

    Centrioles are vital cellular structures that organize centrosomes and cilia. Due to their sub-resolutional size, centriole ultrastructural features have been traditionally analyzed by electron microscopy. Here we present an adaptation of magnified analysis of the proteome expansion microscopy method, to be used for a robust analysis of centriole number, duplication status, length, structural abnormalities and ciliation by conventional optical microscopes. The method allows the analysis of centriole's structural features from large populations of adherent and nonadherent cells and multiciliated cultures. We validate the method using EM and super-resolution microscopy and show that it can be used as an affordable and reliable alternative to electron microscopy in the analysis of centrioles and cilia in various cell cultures. This article is protected by copyright. All rights reserved.

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External Sources

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  2. DOI: 10.1111/jmi.12841
  3. PMID: 31691972
  4. View record in Web of Science®
  5. WOS: 000497138300001

Library Notes

  1. Fiscal Year: FY2019-2020
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