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Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED

  1. Author:
    Zhu, Lan
    Bu, Guanhong
    Jing, Liang
    Shi,Dan
    Lee, Ming-Yue
    Gonen, Tamir
    Liu, Wei
    Nannenga, Brent L

  2. Author Address

    Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA; School of Molecular Sciences, Arizona State University, 551 East University Drive, Tempe, AZ 85287, USA., Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA; School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, USA., Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA., Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Departments of Biological Chemistry and Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA., Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA; School of Molecular Sciences, Arizona State University, 551 East University Drive, Tempe, AZ 85287, USA. Electronic address: w.liu@asu.edu., Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA; School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, USA. Electronic address: brent.nannenga@asu.edu.,
    1. Year: 2020
    2. Date: OCT 6
    3. Epub Date: 2020 07 28
  2. Journal: Structure (London, England : 1993)
    1. 28
    2. 10
    3. Pages: 1149-+
  4. Type of Article: Article
  5. ISSN: 0969-2126
  1. Abstract:

    The lipidic cubic phase (LCP) technique has proved to facilitate the growth of high-quality crystals that are otherwise difficult to grow by other methods. However, the crystal size optimization process could be time and resource consuming, if it ever happens. Therefore, improved techniques for structure determination using these small crystals is an important strategy in diffraction technology development. Microcrystal electron diffraction (MicroED) is a technique that uses a cryo-transmission electron microscopy to collect electron diffraction data and determine high-resolution structures from very thin micro- and nanocrystals. In this work, we have used modified LCP and MicroED protocols to analyze crystals embedded in LCP converted by 2-methyl-2,4-pentanediol or lipase, including Proteinase K crystals grown in solution, cholesterol crystals, and human adenosine A2A receptor crystals grown in LCP. These results set the stage for the use of MicroED to analyze microcrystalline samples grown in LCP, especially for those highly challenging membrane protein targets. Copyright © 2020 Elsevier Ltd. All rights reserved.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.str.2020.07.006
  3. PMID: 32735770
  4. View record in Web of Science®
  5. WOS: 000579354700009
  6. PII : S0969-2126(20)30239-2

Library Notes

  1. Fiscal Year: FY2019-2020
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