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Detection of the DNA binding of transcription factors in situ at the single-cell resolution in cultured cells by proximity ligation assay

  1. Author:
    Ram,Babul
    Dai,Chengkai

  2. Author Address

    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA. Electronic address: babul.ram@nih.gov., Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA. Electronic address: chengkai.dai@nih.gov.,
    1. Year: 2023
    2. Date: Nov 01
    3. Epub Date: 2023 11 01
  2. Journal: STAR Protocols
    1. 4
    2. 4
    3. Pages: 102692
  4. Type of Article: Article
  5. Article Number: 102692
  1. Abstract:

    Transcription factors (TFs) play a pivotal role in gene expression, and their DNA binding is the prerequisite to instigating gene transcription. Here, we present a protocol that exploits the proximity ligation assay technique to measure the DNA-binding activities of TFs in situ at the single-cell resolution. We describe steps for immunostaining with specific antibodies against double-stranded DNA and the TFs of interest, probe incubation, proximity ligation, and signal amplification. We then detail procedures for imaging and image analysis. For complete details on the use and execution of this protocol, please refer to Dai et al. (2015)1 and Xu et al. (2023).2. Published by Elsevier Inc.

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External Sources

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  2. DOI: 10.1016/j.xpro.2023.102692
  3. PMID: 37917578
  4. PII : S2666-1667(23)00659-7

Library Notes

  1. Fiscal Year: FY2023-2024
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