Skip NavigationSkip to Content
Return Return to Search Results

Effect of temperature on the separation of DNA fragments by highperformance liquid chromatography and capillary electrophoresis: a comparative study

  1. Author:
    Issaq, H. J.
    Xu, H. Y.
    Chan, K. C.
    Dean, M. C.

  2. Author Address

    Issaq HJ NCI, SAIC Frederick, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, SAIC Frederick, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Journal of Chromatography B
    1. 738
    2. 2
    3. Pages: 243-248
  4. Type of Article: Article
  1. Abstract:

    This study investigates the effect of experimental temperature on the separation of DNA fragments, 21-587 bp, by both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). The results show that the temperature plays an important role in the HPLC separation of DNA fragments. The optimum temperature was found to be between 40 and 50 degrees C for HPLC, while 25 degrees C was the optimum temperature for the CE separation. Also, although CE migration times became shorter, efficiency and resolution decreased with an increase in temperature from 25 to 50 degrees C, but the separation was not significantly affected, Also, the optimum HPLC temperature might be different depending on the fragment sizes to be resolved. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 26]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel