Skip NavigationSkip to Content
Return Return to Search Results

A quantification of human cells using an ERV-3 real time PCR assay

  1. Author:
    Yuan, C. C.
    Miley, W.
    Waters, D.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Viral Epidemiol Sect, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Viral Epidemiol Sect, Frederick, MD 21702 USA. Yuan CC NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Viral Epidemiol Sect, Frederick, MD 21702 USA.
    1. Year: 2001
  2. Journal: Journal of Virological Methods
    1. 91
    2. 2
    3. Pages: 109-117
  4. Type of Article: Article
  1. Abstract:

    A novel approach to quantifying human cells using a real time PCR assay was developed. The target sequence used in the assay is a 135 bp segment within the unique 1.7 kb Hind III; Pst I fragment of the ERV-3 envelope gene. ERV-3 is a full-length human endogenous retrovirus present in known copy number in all human cells. The detection range of ERV-3 by real time PCR is from 10(6) to 10(1). The precision described, sensitivity and specificity of the assay indicate that the ERV-3 sequence is an accurate cell quantitation marker. The quantitative ERV-3 assay enables simple. fast, and reproducible detection and quantitation of the cell number. The assay can be used to determine the sample DNA conditions and also it can be used to adjust the quantitative DNA measurements of other target gene assays relative to the number of cell equivelants, (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel