Skip NavigationSkip to Content
Return Return to Search Results

Expression of hypoxia-inducible carbonic anhydrases in brain tumors

  1. Author:
    Proescholdt, M. A.
    Mayer, C.
    Kubitza, M.
    Schubert, T.
    Liao, S. Y.
    Stanbridge, E. J.
    Ivanov, S.
    Oldfield, E. H.
    Brawanski, A.
    Merrill, M. J.

  2. Author Address

    NINDS, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA. Univ Regensburg, Dept Neurosurg, D-8400 Regensburg, Germany. Univ Regensburg, Inst Pathol, D-8400 Regensburg, Germany. Univ Calif Irvine, Coll Med, Dept Microbiol & Mol Genet, Irvine, CA 92717 USA. SAIC Frederick Inc, Basic Res Program, Immunobiol Lab, NCI, Frederick, MD 21702 USA Merrill, MJ, NINDS, Surg Neurol Branch, NIH, Bldg 36,Rm 5D37,10 Ctr Dr, Bethesda, MD 20892 USA
    1. Year: 2005
    2. Date: OCT
  2. Journal: Neuro-Oncology
    1. 7
    2. 4
    3. Pages: 465-475
  4. Type of Article: Article
  1. Abstract:

    Malignant brain tumors exhibit distinct metabolic characteristics. Despite high levels of lactate, the intracellular pH of brain tumors is more alkaline than normal brain. Additionally, with increasing malignancy, brain tumors display intratumoral hypoxia. Carbonic anhydrase (CA) IX and XII are transmembrane isoenzymes that are induced by tissue hypoxia. They participate in regulation of pH homeostasis by catalyzing the reversible hydration of carbon dioxide. The aim of our study was to investigate whether brain tumors of different histology and grade of malignancy express elevated levels of CA IX and XII as compared to normal brain. We analyzed 120 tissue specimens from brain tumors (primary and metastatic) and normal brain for CA IX and XII expression by immunohistochemistry, Western blot, and in situ hybridization. Whereas normal brain tissue showed minimal levels of CA IX and XII expression, expression in tumors was found to be upregulated with increased level of malignancy. Hemangioblastomas, from patients with von Hippel-Lindau disease, also displayed high levels of CA IX and XII expression. Comparison of CA IX and XII staining with HIF-let staining revealed a similar microanatomical distribution, indicating hypoxia as a major, but not the only, induction factor. The extent of CA IX and XII staining correlated with cell proliferation, as indicated by Ki67 labeling. The results demonstrate that CA IX and XII are upregulated in intrinsic and metastatic brain tumors as compared to normal brain tissue. This may contribute to the management of tumor-specific acid load and provide a therapeutic target

    See More

  1. Keywords:

External Sources

  1. View record in Web of Science®
  2. WOS: 000232514600005

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel