Skip NavigationSkip to Content
Return Return to Search Results

Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies

  1. Author:
    Hansson, S. R.
    Chen, Y.
    Brodszki, J.
    Chen, M.
    Hernandez-Andrade, E.
    Inman, J. M.
    Kozhich, O. A.
    Larsson, I.
    Marsal, K.
    Medstrand, P.
    Xiang, C. C.
    Brownstein, M. J.

  2. Author Address

    Lund Univ, Univ Lund Hosp, Dept Obstet & Gynecol, S-22185 Lund, Sweden. NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. NIMH, Genet Lab, NIH, Bethesda, MD 20892 USA. NCI, SAIC Frederick, Frederick, MD 21701 USA. Lund Univ, Dept Cell & Mol Biol, Lund, Sweden.;Hansson, SR, Lund Univ, Univ Lund Hosp, Dept Obstet & Gynecol, BMC-C14, S-22185 Lund, Sweden.;stefan.hansson@med.lu.se
    1. Year: 2006
    2. Date: Mar
  2. Journal: Molecular Human Reproduction
    1. 12
    2. 3
    3. Pages: 169-179
  4. Type of Article: Article
  5. ISSN: 1360-9947
  1. Abstract:

    The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five with bilateral uterine artery notching in Doppler velocimetry tracings. Human cDNA microarrays with 6500 transcripts/genes were used and the results verified with real-time PCR and in-situ hybridization. Multidimensional scaling method and random permutation technique demonstrated significant differences among the three groups examined. Within the 6.5K arrays, 6198 elements were unique cDNA clones representing 5952 unique UniGenes and 5695 unique LocusLinks. Multidimensional scaling plots showed 5000 genes that met our quality criteria; among these, 366 genes were significantly different in at least one comparison. Differences in three genes of interest were confirmed with real-time PCR and in-situ hybridization; acid phosphatase 5 was shown to be overexpressed in PE samples and calmodulin 2 and v-rel reticuloendotheliosis viral oncogene homolog A (RELA) were downregulated in PE and uterine artery notch placentas. In conclusion downregulation of RELA and calmodulin 2 might represent an attempt by the placenta to compensate for elevations in intracellular calcium, possibly caused by hypoxia and/or apoptosis, in both pregnancies with uterine artery notching and preeclampsia.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1093/molehr/gal011
  3. View record in Web of Science®
  4. WOS: 000236809000005

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel