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Quantitative proteomic analysis of human breast epithelial cells with differential telomere length

  1. Author:
    Yu, L. R.
    Chan, K. C.
    Tahara, H.
    Lucas, D. A.
    Chatterjee, K.
    Issaq, H. J.
    Veenstra, T. D.

  2. Author Address

    NCI, Lab Proteom & Analyt Technol, SAIC Frederick, Frederick, MD 21702 USA. Hiroshima Univ, Dept Cellular & Mol Biol, Hiroshima 7348551, Japan.;Yu, LR, NCI, Lab Proteom & Analyt Technol, SAIC Frederick, Frederick, MD 21702 USA.;lyu@ncifcrf.gov veenstra@ncifcrf.gov
    1. Year: 2007
    2. Date: May
  2. Journal: Biochemical and Biophysical Research Communications
    1. 356
    2. 4
    3. Pages: 942-947
  4. Type of Article: Article
  5. ISSN: 0006-291X
  1. Abstract:

    Telomeres play important functional roles in cell proliferation, cell cycle regulation, and genetic stability, in which telomere length is critical. In this study, quantitative proteome comparisons for the human breast epithelial cells with short and long telomeres (hTERT(L) vs. 184-hTERT(S) and 90P-hTERT(L) vs. 90P-hTERT(S)), resulting from transfection of the human telomerase reverse transcriptase (hTERT) gene, were performed using cleavable isotope-coded affinity tags. More than 2000 proteins were quantified in each comparative experiment, with approximately 77% of the proteins identified in both analyses. In the cells with long telomeres, significant and consistent alterations were observed in metabolism (amino acid, nucleotide, and lipid metabolism), genetic information transmission (transcription and translation regulation, spliceosome and ribosome complexes), and cell signaling. Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions. (c) 2007 Elsevier Inc. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.bbrc.2007.03.069
  3. View record in Web of Science®
  4. WOS: 000245833500019

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