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Overexpression of Phosphoinositide-Specific Phospholipase C-Gamma in Nih 3t3 Cells Promotes Transformation and Tumorigenicity

  1. Author:
    Smith, M. R.
    Court, D. W.
    Kim, H. K.
    Park, J. B.
    Rhee, S. G.
    Rhim, J. S.
    Kung, H. F.
    1. Year: 1998
  2. Journal: Carcinogenesis
    1. 19
    2. 1
    3. Pages: 177-185
  4. Type of Article: Article
  1. Abstract:

    Phosphoinositide-specific phospholipase C gamma (PLC gamma) is a key regulatory enzyme that binds to the phosphoryl-tyrosine residues in the cytoplasmic domain of certain activated receptors and catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] forming IP3 and diacylglycerol (DAG) in response to several mitogenic factors, Previously, me determined that microinjected PLC gamma induces DNA synthesis in G(0)-arrested NIH 3T3 cells, suggesting the possibility that PLC gamma mag have an oncogenic potential, In this report, we demonstrate that overexpression of PLC gamma in NIH 3T3 cells results in altered growth properties and cellular transformation, The PLC gamma/3T3 transfectants do not require serum growth factors to proliferate, display anchorage-independent growth in soft agar and induce tumors when transplanted into nude mice. These findings suggest that overexpression of PLC gamma facilitates the transformation of NIH 3T3 cells. Furthermore, PLC gamma expression and activity have been shown to be elevated in many human tumors, Thus, PLC gamma signaling may contribute to the promotion and/or progression of human cancers. [References: 55]

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