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Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane-embedded probe reveals insertion of portions of the HIV-1 gp41 cytoplasmic tail into the viral membrane

  1. Author:
    Viard, M.
    Ablan, S. D.
    Zhou, M.
    Veenstra, T. D.
    Freed, E. O.
    Raviv, Y.
    Blumenthal, R.

  2. Author Address

    Viard, Mathias, Raviv, Yossef, Blumenthal, Robert] Natl Inst Hlth, Natl Canc Inst, Ctr Canc Res Nanobiol Program, Frederick, MD 21702 USA. [Viard, Mathias, Raviv, Yossef] Natl Inst Hlth, Natl Canc Inst, Basic Res Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Ablan, Sherimay D.; Freed, Eric O.] Natl Inst Hlth, Natl Canc Inst, Virus Interaction Sect HIV Drug Resistance Progra, Frederick, MD 21702 USA. [Zhou, Ming, Veenstra, Timothy D.] Natl Inst Hlth, Natl Canc Inst, Adv Technol Program, Lab Proteom & Analyt Technol, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Biochemistry
    1. 47
    2. 7
    3. Pages: 1977-1983
  4. Type of Article: Article
  1. Abstract:

    The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated. To examine portions of membrane proteins that are embedded in membrane lipids, we have studied photoinduced chemical reactions in membranes using the lipid bilayer specific probe iodonaphthyl azide (INA). Here we show that in addition to the transmembrane anchor, amphipatic sequences in the cytoplasmic tail (CT) of HIV-1 gp41 are labeled by INA. INA labeling of the HIV-1 gp41 CT was similar whether wild-type or a mutant HIV-1 was used with uncleaved p55 Gag, which does not allow entry. These results shed light on the disposition of the HIV-1 gp41 CT with respect to the membrane. Moreover, our data have general implications for topology of membrane proteins and their in situ interactions with the lipid bilayer.

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External Sources

  1. PMID: 18198900

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