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Enhanced stem cell tracking via electrostatically assembled fluorescent SPION-peptide complexes

  1. Author:
    Lee, J. H.
    Smith, M. A.
    Liu, W.
    Gold, E. M.
    Lewis, B.
    Song, H. T.
    Frank, J. A.

  2. Author Address

    Lee, Jae-Ho, Smith, Melissa A.; Liu, Wei, Gold, Eric M.; Lewis, Bobbi, Song, Ho-Taek, Frank, Joseph A.] NIH, Frank Lab, Ctr Clin, Bethesda, MD 20892 USA. [Lee, Jae-Ho] Natl Canc Inst Hlth Frederick, CCR Nanobiol Program, Membrane Struct & Funct Sect, Frederick, MD 21702 USA. [Liu, Wei] Philips Res N Amer, Briarcliff Manor, NY 10510 USA. [Song, Ho-Taek] Yonsei Univ, Coll Med, Dept Radiol, Seoul 120752, South Korea. [Frank, Joseph A.] Natl Inst Biomed Imaging & Bioengn, Intramural Res Program, NIH, Bethesda, MD 20892 USA.
    1. Year: 2009
  2. Journal: Nanotechnology
    1. 20
    2. 35
  4. Type of Article: Article
  5. Article Number: 355102
  1. Abstract:

    For cellular MRI there is a need to label cells with superparamagnetic iron oxide nanoparticles (SPION) that have multiple imaging moieties that are nontoxic and have increased NMR relaxation properties to improve the detection and tracking of therapeutic cells. Although increases in the relaxation properties of SPION have been accomplished, detection of tagged cells is limited by either poor cell labeling efficiency or low intracellular iron content. A strategy via a complex formation with transfection agents to overcome these obstacles has been reported. In this paper, we report a complex formation between negatively charged fluorescent monodisperse SPION and positively charged peptides and use the complex formation to improve the MR properties of labeled stem cells. As a result, labeled stem cells exhibited a strong fluorescent signal and enhanced T2*-weighted MR imaging in vitro and in vivo in a flank tumor model.

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  2. DOI: 10.1088/0957-4484/20/35/355102
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