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Methods for the analysis of HIV-1 nucleocapsid protein interactions with oligonucleotides

  1. Author:
    Stephen, A. G.
    Fisher, R. J.
    1. Year: 2009
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 485
    2. Pages: 209-221
  4. Type of Article: Article
  1. Abstract:

    HIV-1 Nucleocapsid protein (NC) is a small basic protein that contains two retroviral zinc fingers. It is a highly effective nucleic acid chaperone that plays a critical role in viral replication acting as a cofactor in reverse transcription as well as other aspects of the viral lifecycle. We have used a variety of biophysical techniques to characterize the high affinity binding of NC to a short deoxyoligonucleotide (d(TG)(4)). Here we outline in detail the use of fluorescence anisotropy and surface plasmon resonance spectroscopy to study the binding of NC to d(TG)(4).

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External Sources

  1. PMID: 19020828

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