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Molecular Cloning of Fkhrl1p2, a Member of the Developmentally Regulated Fork Head Domain Transcription Factor Family

  1. Author:
    Dasilva, L.
    Kirken, R. A.
    Taub, D. D.
    Evans, G. A.
    Duhe, R. J.
    Bailey, M. A.
    Farrar, W. L.

  2. Author Address

    Dasilva L SAIC FREDERICK IRSP DIV BASIC SCI FREDERICK, MD 21702 USA NIA IMMUNOL LAB GRC NIH BALTIMORE, MD 21224 USA NCI FREDERICK CANC RES & DEV CTR MOL IMMUNOREGULAT LAB CYTOKINE MOL MECH SECT FREDERICK, MD 21702 USA
    1. Year: 1998
  2. Journal: Gene
    1. 221
    2. 1
    3. Pages: 135-142
  4. Type of Article: Article
  1. Abstract:

    Here we report the expression of a fork head domain protein in human T helper cells. We cloned and characterized a fork head cDNA from human T helper cell mRNA using differential display RT-PCR. The cDNA contains a 546-nucleotide (nt) open reading frame (ORF) that codes for the carboxyl-terminal 180 amino acids (aa) of the recently identified fkhrl1 gene. This ORF does not contain the characteristic DNA-binding domain found in members of the forkhead protein family. In-vitro transcription/translation of this cDNA expressed a protein of approximately 20 kDa. We have generated antibodies that specifically immunoprecipitated the in-vitro-translated 20-kDa protein. This antibody also recognizes in human T lymphocytes a 70-kDa protein corresponding in size to that predicted for the fkhrl1 gene product. The mRNA levels for fkhrl1 is elevated in T helper-induced lymphocytes in comparison to PHA-stimulated T lymphocytes. Further characterization of FKHRL1 and its related family members should shed light on the transcriptional mechanisms of this fork head gene subfamily and their role in T helper cell differentiation and regulation of cell growth. (C) 1998 Elsevier Science B.V. All rights reserved. [References: 26]

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