Skip NavigationSkip to Content
Return Return to Search Results

Schizosaccharomyces pombe Dss1p Is a DNA Damage Checkpoint Protein That Recruits Rad24p, Cdc25p, and Rae1p to DNA Double-strand Breaks

  1. Author:
    Selvanathan, S. P.
    Thakurta, A. G.
    Dhakshnamoorthy, J.
    Zhou, M.
    Veenstra, T. D.
    Dhar, R.

  2. Author Address

    [Selvanathan, Saravana P.; Thakurta, Anjan G.; Dhakshnamoorthy, Jothy; Dhar, Ravi] NCI, Basic Res Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Zhou, Ming; Veenstra, Timothy D.] NCI, Lab Prote & Analyt Technol, Adv Technol Program, Sci Applicat Int Corp Frederick Inc,NIH, Frederick, MD 21702 USA.;Dhar, R, NCI, Basic Res Lab, Ctr Canc Res, NIH, Bldg 37,Rm 5016,37 Convent Dr,9000 Rockville Pike, Bethesda, MD 20892 USA.;dharr@mail.nih.gov
    1. Year: 2010
    2. Date: May
  2. Journal: Journal of Biological Chemistry
    1. 285
    2. 19
    3. Pages: 14122-14133
  4. Type of Article: Article
  5. ISSN: 0021-9258
  1. Abstract:

    Schizosaccharomyces pombe Dss1p and its homologs function in multiple cellular processes including recombinational repair of DNA and nuclear export of messenger RNA. We found that Tap-tagged Rad24p, a member of the 14-3-3 class of proteins, co-purified Dss1p along with mitotic activator Cdc25p, messenger RNA export/cell cycle factor Rae1p, 19 S proteasomal factors, and recombination protein Rhp51p (a Rad51p homolog). Using chromatin immunoprecipitation, we found that Dss1p recruited Rad24p and Rae1p to the double-strand break (DSB) sites. Furthermore, Cdc25p also recruited to the DSB site, and its recruitment was dependent on Dss1p, Rad24p, and the protein kinase Chk1p. Following DSB, all nuclear Cdc25p was found to be chromatin-associated. We found that Dss1p and Rae1p have a DNA damage checkpoint function, and upon treatment with UV light Delta dss1 cells entered mitosis prematurely with indistinguishable timing from Delta rad24 cells. Taken together, these results suggest that Dss1p plays a critical role in linking repair and checkpoint factors to damaged DNA sites by specifically recruiting Rad24p and Cdc25p to the DSBs. We suggest that the sequestration of Cdc25p to DNA damage sites could provide a mechanism for S. pombe cells to arrest at G(2)/M boundary in response to DNA damage.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1074/jbc.M109.083485
  3. View record in Web of Science®
  4. WOS: 000277299700008

Library Notes

  1. Fiscal Year: FY2009-2010
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel