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Effects of Hapten Density on the Induced Antibody Repertoire

  1. Author:
    Li, Q. A.
    Rodriguez, L. G.
    Farnsworth, D. F.
    Gildersleeve, J. C.

  2. Author Address

    [Li, Qian; Farnsworth, David F.; Gildersleeve, Jeffrey C.] NCI, Biol Chem Lab, NIH, Frederick, MD 21702 USA. [Rodriguez, Luis G.] NCI, Opt Microscopy & Anal Lab, SAIC Frederick Inc, Adv Technol Program, Frederick, MD 21702 USA.;Gildersleeve, JC, NCI, Biol Chem Lab, NIH, Bldg 376, Frederick, MD 21702 USA.
    1. Year: 2010
    2. Date: Aug 16
    3. Epub Date: 7/6/2010
  2. Journal: Chembiochem
    1. 11
    2. 12
    3. Pages: 1686-1691
  4. Type of Article: Article
  5. ISSN: 1439-4227
  1. Abstract:

    Small peptides and oligosaccharides are important antigens for the development of vaccines and the production of monoclonal antibodies Because of their small size, peptides and oligosaccharides are non-immunogenic on their own and typically must be conjugated to a larger carrier protein to elicit an immune response. Selection of a suitable carrier protein, conjugation method, and hapten density are critical for generating an optimal immune response We used a glycan array to compare the repertoire of antibodies induced after immunizing with either low or high-density conjugates of the tumor-associated Tn antigen. At high hapten density, a broader range of antibodies was induced, and reactivity to the clustered Tn antigen was observed. In contrast, antibodies induced by the low-density conjugate had narrower reactivity and did not bind the clustered Tn antigen.

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External Sources

  1. View Full Text
  2. DOI: 10.1002/cbic.201000235
  3. PMID: 20602400
  4. View record in Web of Science®
  5. WOS: 000281538500010

Library Notes

  1. Fiscal Year: FY2009-2010
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