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Comparison of estrogens and estrogen metabolites in human breast tissue and urine

  1. Author:
    Taioli, E.
    Im, A.
    Xu, X.
    Veenstra, T. D.
    Ahrendt, G.
    Garte, S.

  2. Author Address

    [Taioli, Emanuela] Suny Downstate Med Ctr, Brooklyn, NY 11203 USA. [Im, Annie; Ahrendt, Gretchen] Univ Pittsburgh, Div Surg Oncol, Dept Surg, Pittsburgh, PA 15232 USA. [Taioli, Emanuela; Garte, Seymour] Univ Pittsburgh, Grad Sch Publ Hlth, Pittsburgh, PA 15232 USA. [Xu, Xia; Veenstra, Timothy D.] NCI, Lab Prote & Analyt Technol, Adv Technol Program, SAIC Frederick Inc, Frederick, MD 21702 USA.;Taioli, E, Suny Downstate Med Ctr, Brooklyn, NY 11203 USA.;emanuela.taioli@downstate.edu
    1. Year: 2010
    2. Date: Aug
  2. Journal: Reproductive Biology and Endocrinology
    1. 8
    2. Pages: 7
  4. Type of Article: Article
  5. Article Number: 93
  6. ISSN: 1477-7827
  1. Abstract:

    Background: An important aspect of the link between estrogen and breast cancer is whether urinary estrogen levels are representative of the intra-tissue levels of bioavailable estrogens. Methods: This study compares 15 estrogen and estrogen metabolite levels in breast tissue and urine of 9 women with primary breast cancer using a quantitative liquid chromatography-mass spectrometry method. Results: The average levels of estrogens (estrone, 17 beta-estradiol) were significantly higher in breast tissue than in urine. Both the 2 and the 16-hydroxylation pathways were less represented in breast tissue than urine; no components of the 4-hydroxypathway were detected in breast tissue, while 4-hydroxyestrone was measured in urine. However, the 2/16 ratio was similar in urine and breast tissue. Women carrying the variant CYP1B1 genotype (Leu/Val and Val/Val) showed significantly lower overall estrogen metabolite, estrogen, and 16-hydroxylation pathway levels in breast tissue in comparison to women carrying the wild type genotype. No effect of the CYP1B1 polymorphism was observed in urinary metabolites. Conclusions: The urinary 2/16 ratio seems a good approximation of the ratio observed in breast tissue. Metabolic genes may have an important role in the estrogen metabolism locally in tissues where the gene is expressed, a role that is not readily observable when urinary measurements are performed.

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External Sources

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  2. DOI: 10.1186/1477-7827-8-93
  3. View record in Web of Science®
  4. WOS: 000282555900001

Library Notes

  1. Fiscal Year: FY2009-2010
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