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Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

  1. Author:
    Jain, L.
    Gardner, E. R.
    Venitz, J.
    Giaccone, G.
    Houk, B. E.
    Figg, W. D.

  2. Author Address

    [Figg, William D.] NCI, Med Oncol Branch, CCR, NIH, Bethesda, MD 20892 USA. [Jain, Lokesh; Figg, William D.] NCI, Clin Pharmacol Program, Ctr Canc Res, Bethesda, MD 20892 USA. [Gardner, Erin R.] NCI, Clin Pharmacol Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Jain, Lokesh; Venitz, Juergen; Figg, William D.] Virginia Commonwealth Univ, Dept Pharmaceut, Sch Pharm, Richmond, VA 23298 USA. [Houk, Brett E.] Pfizer Inc, Dept Clin Pharmacol, San Diego, CA 92121 USA.;Figg, WD, NCI, Med Oncol Branch, CCR, NIH, 9000 Rockville Pike,Bldg 10,Room 5A01, Bethesda, MD 20892 USA.;wdfigg@helix.nih.gov
    1. Year: 2010
    2. Date: Nov
  2. Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
    1. 878
    2. 30
    3. Pages: 3187-3192
  4. Type of Article: Article
  5. ISSN: 1570-0232
  1. Abstract:

    A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma. following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4 mL of methanol containing internal standard (PF-04972487) to 50 mu L volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex (R) Luna C18(2)(2.0mm x 50 mm, 5 mu m) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25 mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1 -> 350.1 (PF-04928473) and m/z 447.0 -> 329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85 min, respectively. Calibration curves were generated in the range of 2-2000 ng/mL. The accuracy and precision ranged from 94.1 to 99.0% and 86.7 to 97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days. Published by Elsevier B.V.

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External Sources

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  2. DOI: 10.1016/j.jchromb.2010.09.017
  3. View record in Web of Science®
  4. WOS: 000284672300021

Library Notes

  1. Fiscal Year: FY2010-2011
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