Comparative Genomic Hybridization (CGH)

Assessment of chromosome loss, gain or amplification in a sample of genomic DNA by competitive hybridization of two genomes against a target cell.

Testing Options
Identifies chromosomal aberrations  
Translocations (balanced)
No
Translocations (unbalanced)
Yes
Copy number changes
Yes
Additions (>10MB)
Yes
Deletions (>10MB)
Yes
Deletions of specific genes/loci
Sometimes
Inversions
No
Insertions
No
Identifies presence of markers
Sometimes
Distinguishes between double minutes and homogeneously staining regions
No
Identifies chromosomal origins
Sometimes
Identifies specific p/q arms/bands
Sometimes
Requires specifically labeled probes
Yes
More than two probes at one time
Yes
Requires viable material
No
Requires metaphase spreads
Yes
Applicable to interphase nuclei
No
Applicable to DNA extracted from paraffin embedded tissue
Yes
Affected by normal tissue contamination
Yes
Identifies tumor heterogeneity
No
Applicable for studies in other species
Yes

Sample Requirements:

  • DNA: Please send the DNA at 4 degrees C. 250ug/ml DNA Stock at a volume of 50ul per sample.

Please inquire if your sample varies from the listed recommendations. Please inquire for likely turnaround time.