Comparative Molecular Cytogenetics Core Facility
Comparative Genomic Hybridization (CGH)
Assessment of chromosome loss, gain or amplification in a sample of genomic DNA
by competitive hybridization of two genomes against a target cell.
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Testing Options
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Identifies chromosomal aberrations
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Translocations (balanced)
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No
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Translocations (unbalanced)
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Yes
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Copy number changes
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Yes
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Additions (>10MB)
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Yes
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Deletions (>10MB)
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Yes
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Deletions of specific genes/loci
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Sometimes
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Inversions
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No
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Insertions
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No
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Identifies presence of markers
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Sometimes
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Distinguishes between double minutes and homogeneously staining regions
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No
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Identifies chromosomal origins
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Sometimes
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Identifies specific p/q arms/bands
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Sometimes
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Requires specifically labeled probes
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Yes
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More than two probes at one time
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Yes
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Requires viable material
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No
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Requires metaphase spreads
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Yes
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Applicable to interphase nuclei
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No
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Applicable to DNA extracted from paraffin embedded tissue
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Yes
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Affected by normal tissue contamination
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Yes
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Identifies tumor heterogeneity
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No
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Applicable for studies in other species
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Yes
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Sample Requirements:
- DNA: Please send the DNA at 4 degrees C. 250ug/ml DNA Stock at a volume of 50ul
per sample.
Please inquire if your sample varies from the listed recommendations. Please inquire
for likely turnaround time.