Karyotyping

Karyotyping (Human)

  • Standard analysis of metaphase chomosomes using G-banding technique.
  • Monitoring of established cell lines using classical and/or molecular cytogenetic techniques to rule out interspecies culture contamination.
  • Characterize tumor related cell lines for chromosome rearrangement.
  • Special studies using a variety of banding techniques including G, C, and R.

Karyotyping (Mammalian)

  • Standard analysis of metaphase chromosomes from a wide variety of species including but not restricted to mouse.
Testing Options
Identifies chromosomal aberrations
 
Translocations (balanced)
Yes 
Translocations (unbalanced)
Yes 
Copy number changes
Yes 
Additions (>10MB)
Yes 
Deletions (>10MB)
Yes 
Deletions of specific genes/loci
No 
Inversions
Yes 
Insertions
Sometimes 
Identifies presence of markers
Yes 
Distinguishes between double minutes and homogeneously staining regions
Yes 
Identifies chromosomal origins
Sometimes 
Identifies specific p/q arms/bands
Yes 
Requires specifically labeled probes
No 
More than two probes at one time
 
Requires viable material
Yes 
Requires metaphase spreads
Yes 
Applicable to interphase nuclei
No 
Applicable to DNA extracted from paraffin embedded tissue
No 
Affected by normal tissue contamination
Yes 
Identifies tumor heterogeneity
Yes 
Applicable for studies in other species
Yes 

Sample Requirements:

  • Peripheral blood: 5-10 mls in Heparin container
  • Culture: Culture must be actively growing with media supplied. A T-75 flask is preferred. Tissue culture flask must not be vented. This prevents spillage and contamination during transit. Do not fill beyond recommendation of flask i.e. (to the absolute top of flask or tube).

Please inquire if your sample varies from the listed recommendations. Please inquire for likely turnaround time.