Comparative Molecular Cytogenetics Core Facility
Karyotyping
Karyotyping (Human)
- Standard analysis of metaphase chomosomes using G-banding technique.
- Monitoring of established cell lines using classical and/or molecular cytogenetic
techniques to rule out interspecies culture contamination.
- Characterize tumor related cell lines for chromosome rearrangement.
- Special studies using a variety of banding techniques including G, C, and R.
Karyotyping (Mammalian)
- Standard analysis of metaphase chromosomes from a wide variety of species including
but not restricted to mouse.
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Testing Options
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Identifies chromosomal aberrations
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Translocations (balanced)
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Yes
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Translocations (unbalanced)
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Yes
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Copy number changes
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Yes
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Additions (>10MB)
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Yes
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Deletions (>10MB)
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Yes
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Deletions of specific genes/loci
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No
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Inversions
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Yes
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Insertions
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Sometimes
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Identifies presence of markers
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Yes
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Distinguishes between double minutes and homogeneously staining regions
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Yes
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Identifies chromosomal origins
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Sometimes
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Identifies specific p/q arms/bands
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Yes
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Requires specifically labeled probes
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No
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More than two probes at one time
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Requires viable material
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Yes
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Requires metaphase spreads
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Yes
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Applicable to interphase nuclei
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No
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Applicable to DNA extracted from paraffin embedded tissue
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No
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Affected by normal tissue contamination
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Yes
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Identifies tumor heterogeneity
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Yes
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Applicable for studies in other species
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Yes
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Sample Requirements:
- Peripheral blood: 5-10 mls in Heparin container
- Culture: Culture must be actively growing with media supplied. A T-75 flask
is preferred. Tissue culture flask must not be vented. This prevents spillage and
contamination during transit. Do not fill beyond recommendation of flask i.e. (to
the absolute top of flask or tube).
Please inquire if your sample varies from the listed recommendations. Please inquire
for likely turnaround time.