Fluidigm Access Array Background
The Fluidigm Access Array is a high-throughput target-enrichment system compatible with all of the major next-generation sequencing instruments. The user can enrich multiple specific targets from a large number of samples all at one time. The system has the throughput benefits of microfluidics, as well as the flexibility and performance of PCR. Particular capabilities include:
- Target Enrichment
- Sample Barcoding for Multiplexed Sequencing
- Sequencing Library Prep Using Amplicon Tagging
Primers amplify and tag PCR products for next-gen sequencing. Simply provide LMT with your regions of interest. The Access Array™ Integrated Fluidic Circuit (IFC) facilitates parallel amplification of 48 unique samples, in effect preparing 48 sequencing libraries, in just a few hours. Every reaction combines both an amplicon tagging and a barcoding (identification) step that enables all 48 samples to be multiplexed at the sequencing step. This powerful chemistry simplifies the up-front preparation and maximizes the utility of today’s next generation sequencers.
The Fluidigm Access Array System can be used with any PCR-based sample preparation method and with the reagents and primers of your choice. The system includes Access Array IFCs, two IFC Controller AXs, and the Stand-Alone Thermal Cycler. These components deliver sample throughput and coverage scalable with laboratory needs. Additional background information about the technology behind 454 sequencing can be found at http://www.fluidigm.com/access-array-system.html. For more information on this service and its applications to your research, visit our Fluidigm Access Array Protocols and Resources page.